Miller Christopher N, Panagos Charalampos G, Mosedale William R T, Kváč Martin, Howard Mark J, Tsaousis Anastasios D
1Laboratory of Molecular & Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury, UK.
2Biomolecular NMR Facility, School of Biosciences, University of Kent, Canterbury, UK.
Gut Pathog. 2019 Apr 3;11:13. doi: 10.1186/s13099-019-0293-x. eCollection 2019.
is an important gut microbe whose contributions towards infant and immunocompromise patient mortality rates are steadily increasing. Over the last decade, we have seen the development of various tools and methods for studying infection and its interactions with their hosts. One area that is sorely overlooked is the effect infection has on host metabolic processes.
Using a H nuclear magnetic resonance approach to metabolomics, we have explored the nature of the mouse gut metabolome as well as providing the first insight into the metabolome of an infected cell line. Statistical analysis and predictive modelling demonstrated new understandings of the effects of a infection, while verifying the presence of known metabolic changes. Of note is the potential contribution of host derived taurine to the diarrhoeal aspects of the disease previously attributed to a solely parasite-based alteration of the gut environment, in addition to other metabolites involved with host cell catabolism.
This approach will spearhead our understanding of the -host metabolic exchange and provide novel targets for tackling this deadly parasite.
是一种重要的肠道微生物，其对婴儿和免疫功能低下患者死亡率的影响正在稳步上升。在过去十年中，我们看到了用于研究感染及其与宿主相互作用的各种工具和方法的发展。一个被严重忽视的领域是感染对宿主代谢过程的影响。
使用氢核磁共振代谢组学方法，我们探索了小鼠肠道代谢组的性质，并首次深入了解了受感染细胞系的代谢组。统计分析和预测模型展示了对感染影响的新认识，同时验证了已知代谢变化的存在。值得注意的是，宿主来源的牛磺酸除了与宿主细胞分解代谢有关的其他代谢物外，对该疾病腹泻方面的潜在贡献，此前该疾病腹泻方面被认为完全是由寄生虫引起的肠道环境改变所致。
这种方法将引领我们对宿主代谢交换的理解，并为应对这种致命寄生虫提供新的靶点。
