Webb Joshua N, Langille Erin, Hafezi Farhad, Randleman J Bradley, Scarcelli Giuliano
J Refract Surg. 2019 Apr 1;35(4):253-260. doi: 10.3928/1081597X-20190304-01.
To investigate the stiffening effect of localized corneal cross-linking (L-CXL) within and beyond the irradiated region in three dimensions.
Ten porcine eyes were debrided of epithelium and incrementally soaked with 0.1% riboflavin solution. Using a customized, sharp-edged mask, half of the cornea was blocked while the other half was exposed to blue light (447 nm). The three-dimensional biomechanical properties of each cornea were then measured via Brillouin microscopy. An imaging system was used to quantify the optimal transition zone between cross-linked and non-cross-linked sections of the cornea when considering light propagation and scattering.
A broad transition zone of 610 µm in width was observed between the fully cross-linked and non-cross-linked sections, indicating the stiffening response extended beyond the irradiated region. Light propagation and the scattering induced by the riboflavin-soaked cornea accounted for a maximum of 25 and 159 ± 3.2 µm, respectively.
The stiffening effect of L-CXL extends beyond that of the irradiated area. When considering L-CXL protocols clinically, it will be important to account for increased stiffening in surrounding regions. [J Refract Surg. 2019;35(4):253-260.].
在三维空间中研究局部角膜交联（L-CXL）在照射区域内外的强化效果。
对10只猪眼进行上皮清创，并用0.1%核黄素溶液逐步浸润。使用定制的边缘锋利的遮罩，遮挡角膜的一半，同时另一半暴露于蓝光（447nm）下。然后通过布里渊显微镜测量每个角膜的三维生物力学特性。在考虑光传播和散射时，使用成像系统量化角膜交联区和非交联区之间的最佳过渡带。
在完全交联和未交联的区域之间观察到一个宽度为610μm的宽过渡带，表明强化反应超出了照射区域。核黄素浸润角膜引起的光传播和散射分别最多占25μm和159±3.2μm。
L-CXL的强化效果超出了照射区域。在临床考虑L-CXL方案时，考虑周围区域强化增加将很重要。[《屈光手术杂志》。2019；35（4）：253 - 260。]
