CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; University of Chinese Academy of Sciences, Beijing 10049, PR China; Gansu Agriculture Technology College, Lanzhou 730020, PR China.
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China.
Int J Biol Macromol. 2019 Jul 15;133:306-315. doi: 10.1016/j.ijbiomac.2019.04.082. Epub 2019 Apr 13.
Two heteropolysaccharides, BHP-1 and BHP-2, were separated and purified from the edible bulbs of Lilium davidii var. unicolor Cotton by anion-exchange and gel-permeation chromatography. The primary structural characteristics of BHP-1 and BHP-2 were investigated by Congo red test, X-ray diffractometry (XRD) analysis, IR, GC and NMR. The results showed that the weight-average molecular weight (M) and number-average molecular weight (Mn) of BHP-1 and BHP-2 were nearly 1.93 × 10 g/moL, 8.86 × 10 g/moL and 3.52 × 10 g/moL, 2.95 × 10 g/moL, respectively. Glucose and mannose comprised BHP-1 in a relative molar ratio of 5.9:2.0, and BHP-2 was consisted of glucose, galactose, mannose and arabinose with approximate molar ratios of 8.3:1.5:1.0:1.1. Their backbone mainly contained α-(1 → 4)-linked d-glucopyranosyl. In addition, the C-6 of the glucose and C-2 of mannose residue of BHP-1 and BHP-2 were substituted. The two polysaccharides were semi-crystalline substance with multi-branching structure and triple helix conformation. Bioassays showed that BHP-1 and BHP-2 had effective antioxidant activities evaluated by DPPH, scavenging superoxide radical and OH radical in a dose-dependent manner. The two heteropolysaccharides also displayed distinct and selective antibacterial activity against B. cereus, P. putida, M. luteus and K. pneumonia with inhibition zones of 13 mm, 16 mm, 17 mm, 23 mm and 12 mm, 17 mm, 19 mm, 20 mm, respectively. These findings provided a reference for potential applications of the polysaccharides from L. davidii as natural antioxidants and antibacterial agents in food and pharmaceuticals.
从百合科百合属植物黄花百合的食用鳞茎中分离得到两种杂多糖 BHP-1 和 BHP-2，并通过阴离子交换和凝胶渗透色谱进行了分离纯化。通过刚果红试验、X 射线衍射分析（XRD）、红外光谱（IR）、气相色谱（GC）和核磁共振（NMR）等方法对 BHP-1 和 BHP-2 的结构特征进行了研究。结果表明，BHP-1 和 BHP-2 的重均分子量（Mw）和数均分子量（Mn）分别约为 1.93×104g/mol、8.86×104g/mol 和 3.52×104g/mol、2.95×104g/mol。BHP-1 由葡萄糖和甘露糖以摩尔比 5.9:2.0 组成，BHP-2 由葡萄糖、半乳糖、甘露糖和阿拉伯糖以摩尔比 8.3:1.5:1.0:1.1 组成。其糖苷键构型均为β-构型，主链主要由α-(1→4)-连接的 d-吡喃葡萄糖基组成。此外，BHP-1 和 BHP-2 中葡萄糖 C-6 和甘露糖 C-2 发生了取代。两种多糖均为半结晶物质，具有多分支结构和三螺旋构象。生物活性评价结果表明，BHP-1 和 BHP-2 具有良好的抗氧化活性，可通过 DPPH、超氧自由基和 OH 自由基清除实验表现出来，且呈剂量依赖性。此外，两种杂多糖对蜡样芽孢杆菌、铜绿假单胞菌、藤黄微球菌和肺炎克雷伯菌均具有明显的选择性抑菌活性，抑菌圈直径分别为 13mm、16mm、17mm、23mm 和 12mm、17mm、19mm、20mm。该研究为黄花百合多糖作为天然抗氧化剂和抗菌剂在食品和医药领域的潜在应用提供了参考。
