Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen Macarena, Sevilla, Spain; Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena/CSIC/Universidad de Sevilla, Sevilla, Spain; Red Española de Investigación en Patología Infecciosa (REIPI RD16/0016), Instituto de Salud Carlos III, Madrid, Spain.
Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen Macarena, Sevilla, Spain; Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena/CSIC/Universidad de Sevilla, Sevilla, Spain; Red Española de Investigación en Patología Infecciosa (REIPI RD16/0016), Instituto de Salud Carlos III, Madrid, Spain.
J Hosp Infect. 2019 Sep;103(1):e92-e96. doi: 10.1016/j.jhin.2019.04.008. Epub 2019 Apr 12.
The aim of this study was to investigate the effect of polyhexanide (polyhexamethylene biguanide)-betaine (PHMB-B) compared with 2% chlorhexidine against biofilms of high-risk and/or multidrug-resistant bacterial clones. The minimum inhibitory concentrations of both biocides were determined by microdilution. The effect of PHMB-B and chlorhexidine on biofilm was evaluated by spectrophotometry and cell viability assays. At commercial concentrations, PHMB-B reduced 24 h, 48 h and 1-week biofilms of all pathogens tested. PHMB-B was more active than 2% chlorhexidine against Gram-negative bacterial 24 h and 48 h biofilms and Gram-positive bacterial 7-day biofilms. In summary, the activity of PHMB-B was superior to that of 2% chlorhexidine in those biofilms.
本研究旨在探讨聚己双胍（聚六亚甲基双胍）-甜菜碱（PHMB-B）与 2%洗必泰相比，针对高风险和/或多药耐药细菌克隆生物膜的效果。通过微量稀释法测定两种杀生物剂的最小抑菌浓度。通过分光光度法和细胞活力测定评估 PHMB-B 和洗必泰对生物膜的影响。在商业浓度下，PHMB-B 减少了所有测试病原体的 24 小时、48 小时和 1 周生物膜。PHMB-B 对革兰氏阴性菌 24 小时和 48 小时生物膜以及革兰氏阳性菌 7 天生物膜的活性均优于 2%洗必泰。综上所述，PHMB-B 的活性优于 2%洗必泰在这些生物膜中的活性。
