Zheng Yaqian, Wang Di, Ma Luyan Z
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2021 Jan 14;22(2):768. doi: 10.3390/ijms22020768.
Hospital-acquired infection is a great challenge for clinical treatment due to pathogens' biofilm formation and their antibiotic resistance. Here, we investigate the effect of antiseptic agent polyhexamethylene biguanide (PHMB) and undecylenamidopropyl betaine (UB) against biofilms of four pathogens that are often found in hospitals, including Gram-negative bacteria and , Gram-positive bacteria , and pathogenic fungus, . We show that 0.02% PHMB, which is 10-fold lower than the concentration of commercial products, has a strong inhibitory effect on the growth, initial attachment, and biofilm formation of all tested pathogens. PHMB can also disrupt the preformed biofilms of these pathogens. In contrast, 0.1% UB exhibits a mild inhibitory effect on biofilm formation of the four pathogens. This concentration inhibits the growth of . and yet has no growth effect on or . UB only slightly enhances the anti-biofilm efficacy of PHMB on biofilms. However, pretreatment with PslG, a glycosyl hydrolase that can efficiently inhibit and disrupt biofilm, highly enhances the clearance effect of PHMB on biofilms. Meanwhile, PslG can also disassemble the preformed biofilms of the other three pathogens within 30 min to a similar extent as UB treatment for 24 h.
由于病原体形成生物膜及其抗生素耐药性，医院获得性感染对临床治疗构成了巨大挑战。在此，我们研究了防腐剂聚六亚甲基双胍（PHMB）和十一烯酰胺丙基甜菜碱（UB）对医院中常见的四种病原体生物膜的影响，这四种病原体包括革兰氏阴性菌 和 、革兰氏阳性菌 以及致病真菌 。我们发现，浓度为0.02%的PHMB（比商业产品浓度低10倍）对所有测试病原体的生长、初始附着和生物膜形成均具有强烈的抑制作用。PHMB还能破坏这些病原体预先形成的生物膜。相比之下，0.1%的UB对这四种病原体的生物膜形成表现出轻微的抑制作用。该浓度抑制了 和 的生长，但对 或 没有生长抑制作用。UB仅略微增强了PHMB对 生物膜的抗生物膜功效。然而，用能有效抑制和破坏 生物膜的糖基水解酶PslG进行预处理，可显著增强PHMB对 生物膜的清除效果。同时，PslG还能在30分钟内将其他三种病原体预先形成的生物膜分解，其程度与UB处理24小时相似。
