Department of Chemistry, Biochemistry and Biophysics, Institute of Pharmaceutical Chemistry, The University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia.
Department of Chemistry, Biochemistry and Biophysics, Institute of Biochemistry, The University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia.
Molecules. 2019 Apr 3;24(7):1317. doi: 10.3390/molecules24071317.
In cancer treatment, the safe delivery of the drug to the target tissue is an important task. 5-fluorouracil (5-FU), the well-known anticancer drug, was encapsulated into the pores of unmodified mesoporous silica SBA-15, as well as silica modified with 3-aminopropyl and cyclohexyl groups. The drug release studies were performed in two different media, in a simulated gastric fluid (pH = 2) and in a simulated body fluid (pH = 7) by RP-UHPLC. The simple and rapid RP-UHPLC method for quantitative determination of 5-fluorouracil released from unmodified and modified mesoporous silica SBA-15 was established on ODS Hypersil C18 column (150 × 4.6 mm, 5 µm) eluted with mobile phase consisted of methanol: phosphate buffer in volume ratio of 3:97 (/). Separation was achieved by isocratic elution. The flow rate was kept at 1 mL/min, the injection volume was set at 20 µL and the column oven temperature was maintained at 25 °C. The effluent was monitored at 268 nm. This paper provides information about the quantitative determination of the released 5-FU from silica. It was found out that larger amount of the drug was released in neutral pH in comparison with the acidic medium. In addition, surface functionalisation of silica SBA-15 influences the release properties of the drug.
在癌症治疗中，将药物安全地递送到靶组织是一项重要任务。5-氟尿嘧啶（5-FU）是一种众所周知的抗癌药物，已被包裹在未经修饰的介孔硅 SBA-15 的孔中和经过 3-氨丙基和环己基修饰的硅中。通过反相超高效液相色谱法（RP-UHPLC）在两种不同的介质中进行了药物释放研究，即在模拟胃液（pH=2）和模拟体液（pH=7）中进行了研究。建立了一种在 ODS Hypersil C18 柱（150×4.6mm，5µm）上用流动相甲醇：磷酸盐缓冲液（体积比为 3：97（/）洗脱的简单快速的反相超高效液相色谱法（RP-UHPLC），用于定量测定从未修饰和修饰的介孔硅 SBA-15 中释放的 5-氟尿嘧啶。通过等度洗脱实现分离。流速保持在 1mL/min，进样体积设定为 20µL，柱箱温度保持在 25°C。在 268nm 处监测流出物。本文提供了有关从硅中定量测定释放的 5-FU 的信息。结果发现，与酸性介质相比，在中性 pH 下释放出了更多的药物。此外，硅 SBA-15 的表面功能化会影响药物的释放特性。
