乙二胺四乙酸诱导的牛血清白蛋白-金纳米簇自组装
EDC-Induced Self-Assembly of BSA-Au NCs.
作者信息
Zhu Wenli, Li Huili, Wan Ajun
机构信息
Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, and School of Pharmacy, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai, 200240, China.
Institute of New Rural Development, Tong Ji University, Shanghai, 200092, China.
出版信息
J Fluoresc. 2019 May;29(3):627-630. doi: 10.1007/s10895-019-02369-1. Epub 2019 Apr 15.
In this paper, the Au nanoplate with bright red fluorescence was synthesized on the basis of bovine serum albumin stabilized Au nanoclusters (BSA-Au NCs). The small molecule N-ethyl-N'-(dimethylaminopropyl) carbodiimide (EDC) was used as the cross-linking agent to activate the carboxyl group on the surface of BSA, and then the condensation with amino acid in BSA induced the self-assembly of BSA-Au NCs. The particle size of Au nanoplate increased with EDC concentration. In addition, when EDC concentration was 0.0050 M, the Au nanoplate changed from smooth to grainy structure. The Au nanoplate remained the unique fluorescence characteristics of BSA-Au NCs. As EDC concentration reached 0.0025 M, the Au nanoplate even exhibited brighter red emission than that of BSA-Au NCs.
在本文中，基于牛血清白蛋白稳定的金纳米团簇（BSA-Au NCs）合成了具有亮红色荧光的金纳米片。小分子N-乙基-N'-(二甲基氨基丙基)碳二亚胺（EDC）用作交联剂，以活化BSA表面的羧基，然后与BSA中的氨基酸缩合诱导BSA-Au NCs的自组装。金纳米片的粒径随EDC浓度的增加而增大。此外，当EDC浓度为0.0050 M时，金纳米片从光滑结构转变为颗粒状结构。金纳米片保留了BSA-Au NCs独特的荧光特性。当EDC浓度达到0.0025 M时，金纳米片甚至表现出比BSA-Au NCs更亮的红色发射。
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