Clean Energy Research Center, Korea Institute of Science and Technology, 5 Hwarang-ro 14-gil, Seongbuk-gu, Seoul, 02792, Republic of Korea.
Department of Chemical and Biological Engineering, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, Republic of Korea.
Curr Microbiol. 2019 Jun;76(6):732-737. doi: 10.1007/s00284-019-01685-w. Epub 2019 Apr 16.
Several bioprocessing technologies, such as separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and consolidated bioprocessing (CBP), have been highlighted to produce bio-based fuels and chemicals from lignocellulosic biomass. Successful CBP, an efficient and economical lignocellulosic biorefinery process compared with other processes, requires microorganisms with sufficient cellulolytic activity and biofuel/chemical-producing ability. Here, we report the complete genome of Paenibacillus sp. CAA11, a newly isolated promising microbial host for CBP-producing ethanol and organic acids from cellulose. The genome of Paenibacillus sp. CAA11 comprises one 4,888,410 bp chromosome with a G + C content of 48.68% containing 4418 protein-coding genes, 102 tRNA genes, and 39 rRNA genes. The functionally active cellulase, encoded by CAA_GH5 was identified to belong to glycosyl hydrolase family 5 (GH5) and consisted of a catalytic domain and a cellulose-binding domain 3 (CBM3). When cellulolytic activity of CAA_GH5 was assayed through Congo red method by measuring the size of halo zone, the recombinant Bacillus subtilis RIK1285 expressing CAA_GH5 showed a comparable cellulolytic activity to B. subtilis RIK1285 expressing Cel5, a previously verified powerful bacterial cellulase. This study demonstrates the potential of Paenibacillus sp. CAA11 as a CBP-enabling microbe for cost-effective biofuels/chemicals production from lignocellulosic biomass.
几种生物加工技术，如单独水解和发酵 (SHF)、同步糖化和发酵 (SSF) 以及整合生物加工 (CBP)，已被强调用于从木质纤维素生物质生产生物基燃料和化学品。与其他工艺相比，成功的 CBP 是一种高效、经济的木质纤维素生物炼制工艺，需要具有足够纤维素酶活性和生物燃料/化学品生产能力的微生物。在这里，我们报告了 Paenibacillus sp. CAA11 的完整基因组，这是一种新分离的有前途的微生物宿主，可用于从纤维素生产 CBP 乙醇和有机酸。Paenibacillus sp. CAA11 的基因组由一条 4,888,410 bp 的染色体组成，G + C 含量为 48.68%，包含 4418 个蛋白质编码基因、102 个 tRNA 基因和 39 个 rRNA 基因。功能活性纤维素酶，由 CAA_GH5 编码，被鉴定为属于糖苷水解酶家族 5 (GH5)，由一个催化结构域和一个纤维素结合结构域 3 (CBM3)组成。通过刚果红法测量晕圈大小来测定 CAA_GH5 的纤维素酶活性时，表达 CAA_GH5 的枯草芽孢杆菌 RIK1285 显示出与表达先前验证的强大细菌纤维素酶 Cel5 的枯草芽孢杆菌 RIK1285 相当的纤维素酶活性。这项研究表明 Paenibacillus sp. CAA11 作为一种 CBP 使能微生物，具有从木质纤维素生物质生产具有成本效益的生物燃料/化学品的潜力。
