Department of Pharmacology, Faculty of Pharmacy, Hamdard University Karachi, Karachi, Pakistan.
Department of Pharmacy, Dow University of Health Sciences, Karachi, Pakistan.
Metab Brain Dis. 2019 Aug;34(4):991-999. doi: 10.1007/s11011-019-00414-3. Epub 2019 Apr 16.
The aim of the study was to evaluate the anticonvulsant and neuroprotective activity of Cocculus laurifolius D.C leaves in albino wistar rats against strychnine induced convulsions. Initially the extract was investigated for acute oral toxicity testing in order to examine any signs of toxicity and mortality. For anticonvulsant activity, the ethanolic extract was evaluated at doses 200 and 400 mg/kg, p.o. against strychnine induced convulsions model, at 1, 7, 15 and 30th day of treatment. Meanwhile, the neuroprotective effect of the extract was investigated via histopathological assessment. Cocculus laurifolius (200 and 400 mg/kg, p.o.) exhibited anticonvulsant activity as indicated by significant delay in the onset of convulsions and time to death after strychnine induced convulsions. Similarly, significant reduction in the duration of convulsions and percentage of mortality was observed by ethanolic extract (200 and 400 mg/kg p.o.) at 1, 7, 15 and 30th day of test sessions. Furthermore, Cocculus laurifolius leaves (200 and 400 mg/kg p.o.) also exhibited neuroprotective effect with considerable preserved neuronal structures and significant decrease in neuronal apoptosis, in comparison with control. The results obtained from the present study indicate that ethanolic extract of Cocculus laurifolius leaves possess potential anticonvulsant and neuroprotective effect against strychnine induced convulsions. Therefore, it can be concluded that Cocculus laurifolius leaves may be a valuable in management of epilepsy, however further studies are required on large number of animals to confirm these findings.
本研究旨在评估葫芦叶的抗惊厥和神经保护活性对士的宁诱导的惊厥的白化 Wistar 大鼠。首先，提取物进行了急性口服毒性试验，以检查任何毒性和死亡率的迹象。对于抗惊厥活性，以 200 和 400 mg/kg，po 的剂量评价乙醇提取物对士的宁诱导的惊厥模型，在治疗的第 1、7、15 和 30 天。同时，通过组织病理学评估研究提取物的神经保护作用。葫芦叶（200 和 400 mg/kg，po）表现出抗惊厥活性，表现为惊厥发作的潜伏期和士的宁诱导的惊厥后死亡时间显著延迟。同样，在第 1、7、15 和 30 天的测试期间，乙醇提取物（200 和 400 mg/kg，po）也观察到惊厥持续时间和死亡率的显著降低。此外，葫芦叶（200 和 400 mg/kg，po）还表现出神经保护作用，与对照组相比，神经元结构得到了相当的保存，神经元凋亡显著减少。本研究的结果表明，葫芦叶的乙醇提取物具有对抗士的宁诱导的惊厥的潜在抗惊厥和神经保护作用。因此，可以得出结论，葫芦叶可能是管理癫痫的有价值的，但需要对大量动物进行进一步研究以证实这些发现。
