Wuya College of Innovation, School of Pharmacy, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
School of Pharmacy, Tongji Medical College , Huazhong University of Science and Technology , Wuhan 430030 , China.
J Agric Food Chem. 2019 May 8;67(18):5147-5158. doi: 10.1021/acs.jafc.9b01195. Epub 2019 Apr 24.
Ganoderma lucidum, as food, tea, dietary supplement, and medicine, is widely used in China and Eastern Asian countries. In order to discover its anti-inflammatory constituents and provide some references for the usage of G. lucidum and G. sinense, two official species in China, the fruiting bodies of G. lucidum were studied, leading to the isolation of six new triterpenoids (1-6) and 27 known analogues (7-33). Compound 4 exhibited the most potent inhibition on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in RAW264.7 macrophage cells. The production of IL-6 and IL-1β, as well as the expression of iNOS, COX-2, and NF-κB were dose-dependently reduced by 4. The phosphorylations of IκBα and IKKβ in LPS-induced macrophage cells were blocked by 4. Therefore, 4 could be used as a potential anti-inflammatory candidate and the total triterpenoids might be developed as value-added functional food for the prevention of inflammation. In combination of previous studies, it should be cautious for the interchangeable usage of G. lucidum and G. sinense.
灵芝，作为食品、茶饮、膳食补充剂和药物，在中国和东亚国家被广泛应用。为了发现其抗炎成分，并为灵芝和中国两种官方物种——密纹灵芝的使用提供一些参考，对灵芝的子实体进行了研究，分离得到了 6 个新的三萜类化合物（1-6）和 27 个已知类似物（7-33）。化合物 4 对脂多糖（LPS）诱导的 RAW264.7 巨噬细胞中一氧化氮（NO）的产生表现出最强的抑制作用。4 能够剂量依赖性地降低 IL-6 和 IL-1β的产生，并降低 iNOS、COX-2 和 NF-κB 的表达。4 还可以阻断 LPS 诱导的巨噬细胞中 IκBα和 IKKβ的磷酸化。因此，4 可以作为一种有潜力的抗炎候选药物，灵芝的总三萜类化合物可能被开发为具有增值功能的预防炎症的食品。综合以往的研究，应谨慎对待灵芝和密纹灵芝的可互换使用。
