State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Science, Kunming, 650201, China; University of the Chinese Academy of Science, Beijing, 100049, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Science, Kunming, 650201, China.
Phytochemistry. 2020 May;173:112256. doi: 10.1016/j.phytochem.2019.112256. Epub 2020 Feb 12.
Ganoderma lucidum is one of the most famous medicinal fungi and is traditional Chinese medicine with various biological activities in Asian countries. To clarify its pharmacodynamic material basis, 15 lanostane triterpenoidswere obtained from the fruiting bodies of G. lucidum, including 8 previously undescribed lanostanoids. Their structures, including absolute configuration, were established based on ultraviolet, infrared, high-resolution electrospray ionisation mass spectrometry, 1D and 2D nuclear magnetic resonance, and X-ray crystallographic analysis. Ganoluciduone A was an unusual octonorlanostane, which was isolated from Ganoderma for the first time. In addition, the anti-inflammatory activities of all isolates were evaluated by observing their inhibitory effects on nitric oxide production in RAW264.7 cells activated by a lipopolysaccharide. Ganoluciduone B exhibited moderate inhibitory activity on nitric oxide production, with an inhibition rate of 45.5% at a concentration of 12.5 μM.
灵芝是最著名的药用真菌之一，是亚洲国家传统的中药，具有多种生物活性。为了阐明其药效物质基础，从灵芝子实体中得到了 15 种羊毛甾烷三萜，包括 8 种以前未描述过的羊毛甾烷。基于紫外、红外、高分辨电喷雾电离质谱、1D 和 2D 核磁共振和 X 射线晶体学分析，确定了它们的结构，包括绝对构型。Ganoluciduone A 是一种不寻常的八角诺兰烷，这是首次从灵芝中分离得到的。此外，通过观察灵芝分离物对脂多糖激活的 RAW264.7 细胞中一氧化氮生成的抑制作用，评估了所有分离物的抗炎活性。Ganoluciduone B 对一氧化氮的产生表现出中等抑制活性，在 12.5 μM 浓度时抑制率为 45.5%。
