College of Pharmacy, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea.
Bioorg Med Chem Lett. 2013 Mar 1;23(5):1428-32. doi: 10.1016/j.bmcl.2012.12.066. Epub 2013 Jan 3.
Four new lanostane triterpenes, butyl lucidenate P (1), butyl lucidenate D(2) (2), butyl lucidenate E(2) (3) and butyl lucidenate Q (4) along with 11 known compounds (5-15) were isolated from the fruiting bodies of Ganoderma lucidum. Their chemical structures were established mainly by 1D and 2D NMR techniques and mass spectrometry. Their anti-inflammatory activity was evaluated against LPS-induced NO production in macrophage RAW 264.7 cells. Compounds 1, 3, 4, 9, 10 and 15 showed inhibitory potency with IC(50) values of 7.4, 6.4, 4.3, 9.4, 9.2 and 4.5 μM, respectively. Compounds 1, 3 and 15 dose-dependently reduced the LPS-induced iNOS expressions. Preincubation of cell with 1, 3 and 15 significantly suppressed LPS-induced expression of COX-2 protein.
从灵芝的子实体中分离得到了 4 个新的羊毛甾烷三萜，丁基灵芝酸 P（1）、丁基灵芝酸 D（2）（2）、丁基灵芝酸 E（2）（3）和丁基灵芝酸 Q（4），以及 11 个已知化合物（5-15）。它们的化学结构主要通过 1D 和 2D NMR 技术和质谱法确定。对它们进行了抗炎活性评价，以检测它们对 LPS 诱导的 RAW 264.7 巨噬细胞中 NO 产生的抑制作用。化合物 1、3、4、9、10 和 15 表现出抑制活性，IC50 值分别为 7.4、6.4、4.3、9.4、9.2 和 4.5 μM。化合物 1、3 和 15 呈剂量依赖性降低 LPS 诱导的 iNOS 表达。细胞预孵育 1、3 和 15 可显著抑制 LPS 诱导的 COX-2 蛋白表达。
