Elbehiry Ayman, Marzouk Eman, Aldubaib Musaad, Moussa Ihab, Abalkhail Adil, Ibrahem Mai, Hamada Mohamed, Sindi Wael, Alzaben Feras, Almuzaini Abdulaziz Mohammad, Algammal Abdelazeem M, Rawway Mohammed
Department of Public Health, College of Public Health and Health Informatics, Qassim University, Al-Bukairiyah, Saudi Arabia.
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
AMB Express. 2022 May 9;12(1):53. doi: 10.1186/s13568-022-01390-1.
Psychrotrophic Pseudomonas is one of the significant microbes that lead to putrefaction in chilled meat. One of the biggest problems in the detection of Pseudomonas is that several species are seemingly identical. Currently, antibiotic resistance is one of the most significant challenges facing the world's health and food security. Therefore, this study was designed to apply an accurate technique for eliminating the identification discrepancy of Pseudomonas species and to study their resistance against various antimicrobials. A total of 320 chicken meat specimens were cultivated, and the isolated bacteria' were phenotypically recognized. Protein analysis was carried out for cultured isolates via Microflex LT. The resistance of Pseudomonas isolates was recorded through Vitek® 2 AST-GN83 cards. Overall, 69 samples were identified as Pseudomonas spp. and included 18 Pseudomonas lundensis (P. lundensis), 16 Pseudomonas fragi (P. fragi), 13 Pseudomonas oryzihabitans (P. oryzihabitans), 10 Pseudomonas stutzeri (P. stutzeri), 5 Pseudomonas fluorescens (P. fluorescens), 4 Pseudomonas putida (P. putida), and 3 Pseudomonas aeruginosa (P. aeruginosa) isolates. Microflex LT identified all Pseudomonas isolates (100%) correctly with a score value ≥ 2.00. PCA positively discriminated the identified isolates into various groups. The antimicrobial resistance levels against Pseudomonas isolates were 81.16% for nitrofurantoin, 71% for ampicillin and ampicillin/sulbactam, 65.22% for cefuroxime and ceftriaxone, 55% for aztreonam, and 49.28% for ciprofloxacin. The susceptibilities were 100% for cefotaxime, 98.55% for ceftazidime, 94.20% for each piperacillin/tazobactam and cefepime, 91.3% for cefazolin. In conclusion, chicken meat was found to be contaminated with different Pseudomonas spp., with high incidence rates of P. lundensis. Microflex LT is a potent tool for distinguishing Pseudomonads at the species level.
嗜冷假单胞菌是导致冷藏肉腐败的重要微生物之一。假单胞菌检测中最大的问题之一是几个菌种看似相同。目前，抗生素耐药性是全球健康和食品安全面临的最重大挑战之一。因此，本研究旨在应用一种准确的技术来消除假单胞菌菌种鉴定差异，并研究它们对各种抗菌药物的耐药性。共培养了320份鸡肉样本，并对分离出的细菌进行了表型鉴定。通过Microflex LT对培养的分离株进行蛋白质分析。通过Vitek® 2 AST-GN83卡片记录假单胞菌分离株的耐药性。总体而言，69个样本被鉴定为假单胞菌属，包括18株伦登假单胞菌（P. lundensis）、16株腐败假单胞菌（P. fragi）、13株栖稻假单胞菌（P. oryzihabitans）、10株施氏假单胞菌（P. stutzeri）、5株荧光假单胞菌（P. fluorescens）、4株恶臭假单胞菌（P. putida）和3株铜绿假单胞菌（P. aeruginosa）分离株。Microflex LT以得分值≥2.00正确鉴定了所有假单胞菌分离株（100%）。主成分分析将鉴定出的分离株积极地区分为不同组。假单胞菌分离株对呋喃妥因的耐药水平为81.16%，对氨苄西林和氨苄西林/舒巴坦为71%，对头孢呋辛和头孢曲松为65.22%，对氨曲南为55%，对环丙沙星为49.28%。对头孢噻肟的敏感性为100%，对头孢他啶为98.55%，对哌拉西林/他唑巴坦和头孢吡肟各为94.20%，对头孢唑林为91.3%。总之，发现鸡肉被不同的假单胞菌属污染，其中伦登假单胞菌的发生率较高。Microflex LT是在菌种水平区分假单胞菌的有力工具。
