Sinimol S, Sarika A R, Jayakumaran Nair A
Department of Biotechnology, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala, 695581, India.
Kerala State Council for Science, Technology and Environment, Sasthra Bhavan, Pattom, Thiruvananthapuram, Kerala, 695004, India.
3 Biotech. 2016 Jun;6(1):7. doi: 10.1007/s13205-015-0318-1. Epub 2015 Dec 31.
The diversity of some of the culturable microorganisms associated with marine flora and fauna collected off Vizhinjam and Mulloor coast of South India was evaluated and their bioactive production potential determined. From a total of 24 bacteria, 4 actinomycetes and 8 fungi isolated from diverse marine sources, five bacterial species-BLM3, BSP2, BCS1, BCS4 and BMA6 showed inhibitory activity against at least one of the tested pathogens viz., Klebsiella pneumonia KU1, Pseudomonas aeruginosa VL3, Salmonella enterica typhimurium MTCC 98, Escherichia coli MTCC 40, Micrococcus luteus MTCC 105, Staphylococcus simulans MTCC 3610, Proteus vulgaris MTCC 426, Vibrio fluvialis, Vibrio sp. P3a and Vibrio sp. P3b. The isolated actinomycetes and fungi did not produce significant inhibition zones against the tested pathogens; however, the macroalgal isolated actinomycetes strain AMA1 produced reddish pigment in Starch Casein medium which remained stable till the stationary phase of growth. The marine sediment isolate BCS4, identified as Bacillus sp. showed wide spectrum of activity against the tested Gram positive bacteria, S. simulans MTCC 3610 and Gram negative bacteria, Proteus vulgaris with zone of inhibitions of 25 and 11 mm respectively. Better extraction of the bioactive compound was obtained with ethyl acetate when compared with methanol, benzene and hexane and TLC analysis revealed the presence of an active compound. The 16SrRNA sequencing confirmed the potent strain belong to Bacillus sp. and hence designated Bacillus sp. BCS4.
对从印度南部维辛贾姆和穆洛尔海岸采集的海洋动植物相关的一些可培养微生物的多样性进行了评估，并测定了它们产生生物活性物质的潜力。从不同海洋来源分离出的总共24株细菌、4株放线菌和8株真菌中，5株细菌——BLM3、BSP2、BCS1、BCS4和BMA6对至少一种受试病原体表现出抑制活性，这些病原体分别是肺炎克雷伯菌KU1、铜绿假单胞菌VL3、鼠伤寒沙门氏菌MTCC 98、大肠杆菌MTCC 40、藤黄微球菌MTCC 105、模仿葡萄球菌MTCC 3610、普通变形杆菌MTCC 426、河流弧菌、弧菌属P3a和弧菌属P3b。分离出的放线菌和真菌对受试病原体没有产生明显的抑菌圈；然而，从大型藻类中分离出的放线菌菌株AMA1在淀粉酪蛋白培养基中产生了红色色素，该色素在生长稳定期之前一直保持稳定。从海洋沉积物中分离出的BCS4被鉴定为芽孢杆菌属，对受试革兰氏阳性菌模仿葡萄球菌MTCC 3610和革兰氏阴性菌普通变形杆菌表现出广泛的活性，抑菌圈分别为25毫米和11毫米。与甲醇、苯和己烷相比，用乙酸乙酯能更好地提取生物活性化合物，薄层色谱分析显示存在一种活性化合物。16SrRNA测序证实该强效菌株属于芽孢杆菌属，因此命名为芽孢杆菌属BCS4。
