Université de Bordeaux, UR Oenologie, MIB, EA 4577, USC 1366 INRA, 33882 Villenave d'Ornon, France.
Food Funct. 2019 May 22;10(5):2573-2582. doi: 10.1039/c9fo00420c.
ε-Viniferin, a resveratrol dimer, is a naturally occurring stilbene that has been studied so far for its potential beneficial effects on human health. Its low water solubility, its photo-sensitivity and its low bioavailability make its applications in the food industry complicated. To overcome these limitations, ε-viniferin was encapsulated in phospholipid-based multi-lamellar liposomes (MLLs) called spherulites or onions. In the best case, an encapsulation efficiency of 58 ± 3% and a bioactive loading of 4.2 ± 0.5% were reached. Encapsulation of ε-viniferin drastically increased its water solubility by more than 5 orders to reach 17.4 g L-1 and provided protection against its UV-induced isomerization. While ε-viniferin was shown to be significantly toxic to Caco-2 intestinal-like cells for concentrations higher than 25 μM, once encapsulated in MLLs, those cells did not experience any mortality even for the highest tested stilbene concentration (100 μM) as revealed by red neutral assay.
ε-白藜芦醇二聚体是一种天然存在的芪类化合物，迄今为止，人们一直在研究其对人类健康的潜在有益作用。由于其低水溶性、光敏感性和低生物利用度，其在食品工业中的应用变得复杂。为了克服这些限制，ε-白藜芦醇二聚体被封装在磷脂基多层脂质体（MLL）中，称为球晶或洋葱。在最佳情况下，达到了 58±3%的包封效率和 4.2±0.5%的生物活性负载。ε-白藜芦醇二聚体的封装使水溶解度提高了 5 个数量级以上，达到 17.4 g/L，并提供了对其 UV 诱导异构化的保护。虽然 ε-白藜芦醇二聚体对浓度高于 25 μM 的 Caco-2 肠样细胞具有显著的毒性，但一旦封装在 MLL 中，即使在最高测试浓度（100 μM）下，这些细胞也没有经历任何死亡，如红色中性测定所揭示的那样。
