Cafaro A, Napolitano M, Zoli V, Pacilli L, Mezzaroma I, De Laurenzi A, Pandolfi F
Blut. 1987 Jan;54(1):43-9. doi: 10.1007/BF00326026.
In the present report we studied the phenotype of peripheral blood mononuclear cells (PBMC) from 25 patients with B-cell chronic lymphocytic leukemia (CLL). Cells from all the cases expressed monoclonal surface immunoglobulins (SmIg), formed rosettes with mouse erythrocytes (MRFC) and were positive with OKB 2 and OKIa monoclonal antibodies. In addition, CCB 1 monoclonal antibody was positive in 17 out of 20, Leu-1 in 18 out of 21 and Leu-8 in 23 out of 25 cases. Double labelling experiments confirmed that the Leu-8 antigen was co-expressed on Leu-1+, CCB2+, HLA-DR+ B-CLL cells. Thus, B-CLL cells generally express the SmIg+, MRFC+, Leu-1+, OKB2+, Leu-8+ phenotype. Since it is known that normal peripheral blood B cells may be divided into two subpopulations according to Leu-8 expression, our data indicate that B-CLL cells originate from the more immature Leu-8+ B-cell subset which will respond to anti-IgM, whereas it reacts poorly to pokeweed mitogen.
在本报告中，我们研究了25例B细胞慢性淋巴细胞白血病（CLL）患者外周血单个核细胞（PBMC）的表型。所有病例的细胞均表达单克隆表面免疫球蛋白（SmIg），与小鼠红细胞形成玫瑰花结（MRFC），并且对OKB 2和OKIa单克隆抗体呈阳性反应。此外，CCB 1单克隆抗体在20例中的17例呈阳性，Leu-1在21例中的18例呈阳性，Leu-8在25例中的23例呈阳性。双重标记实验证实，Leu-8抗原在Leu-1 +、CCB2 +、HLA-DR +的B-CLL细胞上共表达。因此，B-CLL细胞通常表达SmIg +、MRFC +、Leu-1 +、OKB2 +、Leu-8 +表型。由于已知正常外周血B细胞可根据Leu-8表达分为两个亚群，我们的数据表明B-CLL细胞起源于更不成熟的Leu-8 + B细胞亚群，该亚群将对抗IgM产生反应，而对商陆有丝分裂原反应较差。
