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在植物病原真菌大丽轮枝菌中次生代谢物簇的计算机预测和特征分析。

In silico prediction and characterisation of secondary metabolite clusters in the plant pathogenic fungus Verticillium dahliae.

机构信息

Laboratory of Phytopathology, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.

Department of Crops and Agronomy, National Institute of Agricultural Botany, Huntingdon Road, CB3 0LE Cambridge, United Kingdom.

出版信息

FEMS Microbiol Lett. 2019 Apr 1;366(7). doi: 10.1093/femsle/fnz081.

DOI:10.1093/femsle/fnz081
PMID:31004487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6502550/
Abstract

Fungi are renowned producers of natural compounds, also known as secondary metabolites (SMs) that display a wide array of biological activities. Typically, the genes that are involved in the biosynthesis of SMs are located in close proximity to each other in so-called secondary metabolite clusters. Many plant-pathogenic fungi secrete SMs during infection in order to promote disease establishment, for instance as cytocoxic compounds. Verticillium dahliae is a notorious plant pathogen that can infect over 200 host plants worldwide. However, the SM repertoire of this vascular pathogen remains mostly uncharted. To unravel the potential of V. dahliae to produce SMs, we performed in silico predictions and in-depth analyses of its secondary metabolite clusters. Using distinctive traits of gene clusters and the conserved signatures of core genes 25 potential SM gene clusters were identified. Subsequently, phylogenetic and comparative genomics analyses were performed, revealing that two putative siderophores, ferricrocin and TAFC, DHN-melanin and fujikurin may belong to the SM repertoire of V. dahliae.

摘要

真菌是天然化合物的著名生产者，也被称为次生代谢物（SMs），具有广泛的生物活性。通常，参与 SMs 生物合成的基因位于所谓的次生代谢物簇中彼此靠近的位置。许多植物病原真菌在感染过程中会分泌 SMs，以促进疾病的建立，例如作为细胞毒性化合物。黄萎病菌是一种臭名昭著的植物病原体，可感染全球 200 多种宿主植物。然而，这种血管病原体的 SM 成分谱在很大程度上仍未被发现。为了揭示黄萎病菌产生 SMs 的潜力，我们对其次生代谢物簇进行了计算机预测和深入分析。利用基因簇的独特特征和核心基因的保守特征，鉴定出了 25 个潜在的 SM 基因簇。随后进行了系统发育和比较基因组学分析，结果表明，两种假定的铁载体（ferricrocin 和 TAFC）、DHN-黑色素和 fujikurin 可能属于黄萎病菌的 SM 成分谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c3/6502550/22f896f223b6/fnz081fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c3/6502550/ec149eefb1a1/fnz081fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c3/6502550/a5eec2f9f5f5/fnz081fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c3/6502550/12601f2565bd/fnz081fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c3/6502550/e0ca6da1fe15/fnz081fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c3/6502550/22f896f223b6/fnz081fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c3/6502550/ec149eefb1a1/fnz081fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c3/6502550/a5eec2f9f5f5/fnz081fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c3/6502550/12601f2565bd/fnz081fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c3/6502550/e0ca6da1fe15/fnz081fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03c3/6502550/22f896f223b6/fnz081fig5.jpg

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