Amreen Shaiesta, Kumar Pratap, Gupta Priyanka, Rao Pragna
Department of Obstetrics & Gynaecology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Department of Biochemistry, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.
J Hum Reprod Sci. 2019 Jan-Mar;12(1):40-46. doi: 10.4103/jhrs.JHRS_27_17.
The aim was to evaluate the association between oxidative stress with the severity of endometriosis in blood and peritoneal fluid.
This study was a prospective observational study conducted in the department of obstetrics and gynecology in a tertiary center over 2 years in patients with endometriosis requiring laparoscopy.
Patients diagnosed with endometriosis and undergoing laparoscopy for infertility and/or chronic pelvic pain were included in the study. Out of the 64 patients recruited, only 55 patients formed the part of our study. Blood and peritoneal fluid was evaluated for oxidative stress parameters such as glutathione peroxidase, superoxide dismutase (SOD), and lipid peroxides.
The severity of endometriosis was assessed intraoperatively by the revised American Society for Reproductive Medicine scoring in all 55 patients who underwent laparoscopy into minimal ( = 3), mild ( = 7), moderate ( = 32), and severe ( = 17). Median activity of SOD and glutathione peroxidase was lowest in the severe stage of endometriosis, whereas the median activity of lipid peroxide was highest in the severe stage of endometriosis in both blood and peritoneal fluid samples. There was a statistically significant increase in oxidative stress with the severity of endometriosis.
Oxidative stress might play a role in the disease process of endometriosis. Control of oxidative stress can be used as the latest treatment option for the management of endometriosis.
本研究旨在评估血液和腹腔液中氧化应激与子宫内膜异位症严重程度之间的关联。
本研究为前瞻性观察性研究，在一家三级医疗中心的妇产科进行，为期2年，研究对象为需要进行腹腔镜检查的子宫内膜异位症患者。
本研究纳入了被诊断为子宫内膜异位症且因不孕和/或慢性盆腔疼痛而接受腹腔镜检查的患者。在招募的64名患者中，只有55名患者纳入本研究。对血液和腹腔液进行氧化应激参数评估，如谷胱甘肽过氧化物酶、超氧化物歧化酶（SOD）和脂质过氧化物。
在所有55例行腹腔镜检查的患者中，术中通过修订的美国生殖医学学会评分系统评估子宫内膜异位症的严重程度，分为轻微（=3）、轻度（=7）、中度（=32）和重度（=17）。在血液和腹腔液样本中，SOD和谷胱甘肽过氧化物酶的中位活性在子宫内膜异位症重度阶段最低，而脂质过氧化物的中位活性在子宫内膜异位症重度阶段最高。随着子宫内膜异位症严重程度的增加，氧化应激有统计学意义的升高。
氧化应激可能在子宫内膜异位症的疾病过程中起作用。控制氧化应激可作为子宫内膜异位症管理的最新治疗选择。
