Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Materials Science and Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, USA.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA.
Int J Pharm. 2019 Jun 10;564:273-280. doi: 10.1016/j.ijpharm.2019.04.053. Epub 2019 Apr 19.
Nanoparticular system of a model small molecular drug curcumin (CUR) was prepared using a novel method, namely, flash nanocomplexation by hydrogen bonding interactions. The CUR-loaded nanoparticles (NPs) were fabricated by mixing CUR, tannic acid and polyvinyl alcohol (PVA) in aqueous solutions under turbulent condition through a three-inlet confined impinging jet (CIJ) device. Compared to bulk mixing, FNC has the advantage of scalability, reproducibility and without causing the variations by different mixing sequences. Three NPs with different drug loading levels were prepared by tuning the CUR feeding amount. In human prostate cancer PC3 cells, both cellular uptake and cytotoxicity of these NPs were negatively correlated with the drug loading level. These findings indicate that FNC is an easy and feasible method for small molecular drug delivery by hydrogen bonding interactions.
采用氢键相互作用的闪光纳米复合技术，制备了模型小分子药物姜黄素（CUR）的纳米颗粒体系。将 CUR、鞣酸和聚乙烯醇（PVA）混合于水溶液中，在湍流条件下，通过三入口受限冲击射流（CIJ）装置，制备载药纳米颗粒（NPs）。与批量混合相比，FNC 具有可扩展性、重现性的优势，且不会因不同的混合顺序而产生差异。通过调节 CUR 的进料量，制备了三种载药量不同的 NPs。在人前列腺癌细胞 PC3 中，这些 NPs 的细胞摄取和细胞毒性与载药量呈负相关。这些发现表明，FNC 是一种通过氢键相互作用进行小分子药物传递的简单可行的方法。
