Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, 12180.
Protein Sci. 2019 Jul;28(7):1210-1221. doi: 10.1002/pro.3630. Epub 2019 May 22.
Quantitative characterization of protein interactions, both intramolecular and intermolecular, is crucial in understanding the mechanisms and regulation of their function. In recent years, it has become possible to obtain such information on protein systems in live cells, from bacteria to mammalian cell lines. This review discusses recent advances in measuring protein folding, absolute concentration, oligomerization, diffusion, transport, and organization at super-resolution.
定量描述蛋白质相互作用(包括分子内和分子间相互作用)对于理解其功能的机制和调控至关重要。近年来,已经可以在活细胞中获得有关蛋白质系统的此类信息,从细菌到哺乳动物细胞系。本文综述了近年来在超分辨率下测量蛋白质折叠、绝对浓度、寡聚化、扩散、运输和组织的最新进展。