Okatan D Ö, Kulaber A, Kerimoglu G, Odacı E
Department of Histology and Embryology, Faculty of Medicine, Karadeniz Technical University , Trabzon , Turkey.
Biotech Histochem. 2019 Aug;94(6):420-428. doi: 10.1080/10520295.2019.1580767. Epub 2019 Apr 24.
Despite their benefits, technological devices such as cell phones may also have deleterious effects on human health. Considerable debate continues concerning the effects of the electromagnetic field (EMF) emitted during cell phone use on human health. We investigated the effects of exposure to 900 megahertz (MHz) EMF during mid to late adolescence on the rat liver. Control (ContGr), sham (ShmGr) and EMF (EMFGr) groups of female rats were established. We exposed the EMFGr rats daily to 900 MHz EMF on postnatal days 35-59. ShmGr rats underwent sham procedures. No procedure was performed on ContGr rats. Rats were sacrificed on postnatal day 60 and the livers were extracted. One part of the liver was stained with Masson's trichrome or hematoxylin and eosin. The remaining tissue was used to measure oxidative stress markers including malondialdehyde, glutathione, catalase, superoxide dismutase, 8-hydroxydeoxyguanosine (8-OHdG) and nitrotyrosine. Total antioxidant status and total oxidant status were used to calculate the oxidative stress index. We found normal hepatic morphology in the ContGr and ShmGr groups. The EMFGr group exhibited occasional irregularities in the radial arrangement of hepatocytes, cytoplasmic vacuolization, hemorrhage, sinusoid expansion, hepatocyte morphology and edema. Biochemical analysis revealed that 8-OHdG and SOD levels in EMFGr decreased significantly compared to the ContGr and ShmGr groups. Exposure to a continuous 900 MHz EMF for 1 h daily during mid to late adolescence may cause histopathological and biochemical alterations in hepatic tissue.
尽管手机等科技设备有诸多益处，但它们也可能对人类健康产生有害影响。关于手机使用过程中发出的电磁场（EMF）对人类健康的影响，仍存在大量争议。我们研究了青春期中后期暴露于900兆赫（MHz）电磁场对大鼠肝脏的影响。建立了雌性大鼠的对照组（ContGr）、假手术组（ShmGr）和电磁场组（EMFGr）。我们在出生后第35至59天，每天让EMFGr组的大鼠暴露于900 MHz的电磁场中。ShmGr组的大鼠接受假手术。ContGr组的大鼠不进行任何操作。在出生后第60天处死大鼠并取出肝脏。肝脏的一部分用Masson三色染色法或苏木精-伊红染色。其余组织用于测量氧化应激标志物，包括丙二醛、谷胱甘肽、过氧化氢酶、超氧化物歧化酶、8-羟基脱氧鸟苷（8-OHdG）和硝基酪氨酸。用总抗氧化状态和总氧化状态来计算氧化应激指数。我们发现ContGr组和ShmGr组的肝脏形态正常。EMFGr组的肝细胞径向排列偶尔出现不规则、细胞质空泡化、出血、血窦扩张、肝细胞形态改变和水肿。生化分析显示，与ContGr组和ShmGr组相比，EMFGr组的8-OHdG和超氧化物歧化酶水平显著降低。青春期中后期每天持续1小时暴露于900 MHz的电磁场可能会导致肝脏组织出现组织病理学和生化改变。
