Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea.
Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea.
Molecules. 2019 Apr 23;24(8):1595. doi: 10.3390/molecules24081595.
Cutaneous wound healing is a well-orchestrated event in which many types of cells and growth factors are involved in restoring the barrier function of skin. In order to identify whether ginsenosides, the main active components of , promote wound healing, the proliferation and migration activities of 15 different ginsenosides were tested by MTT assay and scratched wound closure assay. Among ginsenosides, gypenoside LXXV (G75) showed the most potent wound healing effects. Thus, this study aimed to investigate the effects of G75 on wound healing in vivo and characterize associated molecular changes. G75 significantly increased proliferation and migration of keratinocytes and fibroblasts, and promoted wound closure in an excision wound mouse model compared with madecassoside (MA), which has been used to treat wounds. Additionally, RNA sequencing data revealed G75-mediated significant upregulation of connective tissue growth factor (CTGF), which is known to be produced via the glucocorticoid receptor (GR) pathway. Consistently, the increase in production of CTGF was confirmed by western blot and ELISA. In addition, GR-competitive binding assay and GR translocation assay results demonstrated that G75 can be bound to GR and translocated into the nucleus. These results demonstrated that G75 is a newly identified effective component in wound healing.
皮肤创伤愈合是一个协调良好的过程，其中许多类型的细胞和生长因子参与恢复皮肤的屏障功能。为了确定人参的主要活性成分人参皂苷是否促进伤口愈合，通过 MTT 检测和划痕封闭试验测试了 15 种不同人参皂苷的增殖和迁移活性。在人参皂苷中，绞股蓝皂苷 LXXV（G75）表现出最强的伤口愈合作用。因此，本研究旨在研究 G75 在体内对伤口愈合的影响，并阐明相关的分子变化。与已用于治疗伤口的积雪草苷（MA）相比，G75 显著增加了角质形成细胞和成纤维细胞的增殖和迁移，并促进了切除伤口小鼠模型中的伤口闭合。此外，RNA 测序数据显示 G75 介导的结缔组织生长因子（CTGF）的显著上调，已知 CTGF 通过糖皮质激素受体（GR）途径产生。Western blot 和 ELISA 也证实了 CTGF 产量的增加。此外，GR 竞争性结合试验和 GR 易位试验结果表明，G75 可以与 GR 结合并转移到核内。这些结果表明，G75 是一种新发现的有效的伤口愈合成分。
