Peng Xue, Xu Jinfeng, Tian Yuan, Liu Wenjun, Peng Bing
Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China.
Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, China.
Food Nutr Res. 2020 Aug 31;64. doi: 10.29219/fnr.v64.4247. eCollection 2020.
Wound complications are a major source of morbidity after cesarean section (CS) and contribute to increased risks in subsequent pregnancies. In the present study, we aim to investigate the wound healing potential of a kind of oligopeptide compound, mainly derived from the marine fish peptides (MFPs), in rats after CS by biomechanical, biochemical, and histological methods.
Eighty-four pregnant Sprague-Dawleyrats were randomly assigned to four groups, namely the control group and 1.1, 2.2, and 4.4 mg/kg MFP groups, respectively. The MFPs or normal saline of the equal volume was intragastrically administered every morning on the second day after CS. On days 5, 10, and 15 after the surgery, seven rats from each group were randomly selected. The samples of skin wound and uterus were harvested and then used for the following experiments and analyses.
Using the CS rat model, this study demonstrated that in the MFP groups, the skin tensile strength, uterine bursting pressure, and hydroxyproline (Hyp) were significantly higher than those in the control group at all three time points ( < 0.05). The formation of collagen and smooth muscle fibers and the expression of CD34 and connective tissue growth factor at the incision site were increasingly observed in the MFP groups ( < 0.05).
MFPs have a great potential to accelerate the process and quality of wound healing in rats after CS.
伤口并发症是剖宫产术后发病的主要原因，并且会增加后续妊娠的风险。在本研究中，我们旨在通过生物力学、生物化学和组织学方法，研究一种主要来源于海洋鱼类肽（MFP）的寡肽化合物对大鼠剖宫产术后伤口愈合的影响。
将84只怀孕的Sprague-Dawley大鼠随机分为四组，即对照组以及1.1、2.2和4.4mg/kg MFP组。在剖宫产术后第二天，每天早晨对大鼠灌胃给予等体积的MFP或生理盐水。在术后第5、10和15天，从每组中随机选取7只大鼠。采集皮肤伤口和子宫样本，然后用于以下实验和分析。
使用剖宫产大鼠模型，本研究表明，在MFP组中，在所有三个时间点，皮肤抗张强度、子宫破裂压力和羟脯氨酸（Hyp）均显著高于对照组（<0.05）。在MFP组中，切口部位胶原蛋白和平滑肌纤维的形成以及CD34和结缔组织生长因子的表达逐渐增加（<0.05）。
MFP具有加速大鼠剖宫产术后伤口愈合进程和提高愈合质量的巨大潜力。
