Department of Gynecology and Obstetrics, Shengjing Hospital Affiliated of China Medical University.
Department of Gynecology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute.
Biosci Trends. 2019 May 12;13(2):204-211. doi: 10.5582/bst.2019.01021. Epub 2019 Apr 24.
Circular RNA (circRNA) is a class of endogenous non-coding RNAs that are closely related to the pathogenesis of many human diseases, particularly cancer. However, the characterization of circRNAs in high-grade serous ovarian cancer (HGSOC) remains unknown. This study aimed to investigate the expression profile of circRNAs in HGSOC. Expression profiles of circRNAs differential expression based on circRNAs High-throughput sequencing were identified in 3 HGSOC specimens and 3 normal ovarian tissues. A total of 710 differentially expressed circRNAs were found (354 expressions up-regulated and 356 expressions down-regulated). CircRNA sequencing data were verified by qRT-PCR in HGSOC tissue and benign ovarian lesions. Differential expression of 7 circRNAs (circRNA385, circRNA2058, circRNA3336, circRNA2606, circRNA1656, circRNA1312 and circRNA7474) in HGSOC tissue was confirmed by qRT-PCR. Among them, circRNA1656 showed the highest fold change. qRT-PCR was used to verify the expression of circRNA1656 in ovarian cancer cell lines. In order to analyze the relationship between circRNA1656 expression and clinical pathological biological characteristics of HGSOC, qRT-PCR was used to verify the expression of circRNA1656 in 60 HGSOC tissues compared with 60 benign ovarian lesions. The expression of circRNA1656 was down-regulated in HGSOC tissues and ovarian cancer cell lines, and correlated with the FIGO stage of HGSOC. circRNA1656 has the potential to serve as a novel tumor marker for HGSOC.
环状 RNA（circRNA）是一类与许多人类疾病的发病机制密切相关的内源性非编码 RNA，尤其是癌症。然而，高级别浆液性卵巢癌（HGSOC）中 circRNA 的特征尚不清楚。本研究旨在研究 HGSOC 中 circRNA 的表达谱。通过对 3 例 HGSOC 标本和 3 例正常卵巢组织的 circRNAs 高通量测序，确定了基于 circRNAs 的表达谱差异表达。发现了 710 个差异表达的 circRNAs（354 个表达上调，356 个表达下调）。在 HGSOC 组织和良性卵巢病变中，通过 qRT-PCR 验证了 circRNA 测序数据。通过 qRT-PCR 验证了 7 个 circRNAs（circRNA385、circRNA2058、circRNA3336、circRNA2606、circRNA1656、circRNA1312 和 circRNA7474）在 HGSOC 组织中的差异表达。其中，circRNA1656 的倍数变化最大。通过 qRT-PCR 验证了 circRNA1656 在卵巢癌细胞系中的表达。为了分析 circRNA1656 表达与 HGSOC 临床病理生物学特征的关系，通过 qRT-PCR 验证了 60 例 HGSOC 组织与 60 例良性卵巢病变中 circRNA1656 的表达。circRNA1656 在 HGSOC 组织和卵巢癌细胞系中表达下调，并与 HGSOC 的 FIGO 分期相关。circRNA1656 有可能成为 HGSOC 的新型肿瘤标志物。
