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一种分枝杆菌噬菌体的定向进化

Directed Evolution of a Mycobacteriophage.

作者信息

Cebriá-Mendoza María, Sanjuán Rafael, Domingo-Calap Pilar

机构信息

Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, 46980 Paterna, Valencia, Spain.

Department of Genetics, Universitat de València, 46100 Burjassot, Valencia, Spain.

出版信息

Antibiotics (Basel). 2019 Apr 25;8(2):46. doi: 10.3390/antibiotics8020046.

DOI:10.3390/antibiotics8020046
PMID:31027152
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6627502/
Abstract

Bacteriophages represent an alternative strategy to combat pathogenic bacteria. Currently, infections constitute a major public health problem due to extensive antibiotic resistance in some strains. Using a non-pathogenic species of the same genus as an experimental model, , here we have set up a basic methodology for mycobacteriophage growth and we have explored directed evolution as a tool for increasing phage infectivity and lytic activity. We demonstrate mycobacteriophage adaptation to its host under different conditions. Directed evolution could be used for the development of future phage therapy applications against mycobacteria.

摘要

噬菌体是对抗病原菌的一种替代策略。目前，由于某些菌株广泛的抗生素耐药性，感染已成为一个重大的公共卫生问题。我们使用同一属的非致病物种作为实验模型，建立了分枝杆菌噬菌体生长的基本方法，并探索了定向进化作为提高噬菌体感染性和裂解活性的工具。我们证明了分枝杆菌噬菌体在不同条件下对其宿主的适应性。定向进化可用于未来针对分枝杆菌的噬菌体治疗应用的开发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edab/6627502/0333f57a5f4d/antibiotics-08-00046-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edab/6627502/59c159f28e29/antibiotics-08-00046-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edab/6627502/e81d7fb9161a/antibiotics-08-00046-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edab/6627502/599b52f9f23e/antibiotics-08-00046-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edab/6627502/77e6dd7456ba/antibiotics-08-00046-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edab/6627502/0333f57a5f4d/antibiotics-08-00046-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edab/6627502/59c159f28e29/antibiotics-08-00046-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edab/6627502/e81d7fb9161a/antibiotics-08-00046-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edab/6627502/599b52f9f23e/antibiotics-08-00046-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edab/6627502/77e6dd7456ba/antibiotics-08-00046-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edab/6627502/0333f57a5f4d/antibiotics-08-00046-g005.jpg

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