Mamanova Lira
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Methods Mol Biol. 2019;1979:3-8. doi: 10.1007/978-1-4939-9240-9_1.
Transcriptomics has been revolutionized by massive throughput RNA-seq. To date, the ongoing decrease in sequencing cost and recent eruption of single-cell related protocols have boosted a demand for single-cell RNA sequencing projects. Although the single-cell RNA-Seq (scRNA-Seq) approach is close to the conventional "bulk" RNA-seq, several features that are unique to scRNA-seq should be taken into consideration in order to obtain high-quality libraries and unbiased sequencing data.In this chapter I give recommendations for setting up the single cell-suitable laboratory environment.
转录组学因大规模高通量RNA测序而发生了变革。迄今为止，测序成本的持续下降以及近期单细胞相关方案的涌现，推动了对单细胞RNA测序项目的需求。尽管单细胞RNA测序（scRNA-Seq）方法与传统的“批量”RNA测序相近，但为了获得高质量文库和无偏倚的测序数据，仍应考虑scRNA-Seq独有的几个特征。在本章中，我将给出关于建立适合单细胞研究的实验室环境的建议。
