Institute of Marine Research, PO Box 1870 Nordnes, N-5817 Bergen, Norway.
Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway.
Nutrients. 2019 Apr 27;11(5):966. doi: 10.3390/nu11050966.
Although the correlation between polyunsaturated fatty acids (PUFA) and the production of pro- and anti-inflammatory metabolites is well documented, little is known about the simultaneous effect of different PUFA on the production of cyclooxygenase and lipoxygenase metabolites. The present research examines the association between different omega-3 (ω-3) and omega-6 (ω-6) PUFA and the release of four cyclooxygenase and six lipoxygenase metabolites in cell medium by human umbilical vein endothelial cells (HUVEC). The different combinations of ω-3 and ω-6 PUFA were prepared according to a full 2 factorial design that enables studying not only the main effects but also the different interactions between fatty acids. In addition, interactions diagrams and principal component analysis were useful tools for interpreting higher order interactions. To the best of our knowledge, this is the first report addressing the combined effect of ω-3 and ω-6 PUFA on the signaling of prostaglandins, prostacyclins, leukotrienes and resolvins by HUVEC.
尽管多不饱和脂肪酸（PUFA）与前炎症和抗炎代谢产物的产生之间存在相关性已得到充分证实，但对于不同 PUFA 对环氧化酶和脂氧化酶代谢产物产生的同时影响，人们知之甚少。本研究考察了不同的ω-3（ω-3）和 ω-6（ω-6）PUFA 与人类脐静脉内皮细胞（HUVEC）细胞培养基中四种环氧化酶和六种脂氧化酶代谢产物释放之间的关系。根据完全 2 因子设计制备不同的 ω-3 和 ω-6 PUFA，不仅可以研究主要效应，还可以研究脂肪酸之间的不同相互作用。此外，交互图和主成分分析是解释高阶交互作用的有用工具。据我们所知，这是首次报道 ω-3 和 ω-6 PUFA 对 HUVEC 中前列腺素、前列环素、白三烯和 resolvins 信号的联合作用。
