Furley A J, Mizutani S, Weilbaecher K, Dhaliwal H S, Ford A M, Chan L C, Molgaard H V, Toyonaga B, Mak T, van den Elsen P
Cell. 1986 Jul 4;46(1):75-87. doi: 10.1016/0092-8674(86)90861-5.
Human leukemic cells corresponding to the earliest identifiable stages of intrathymic T cell differentiation lack cell surface expression of the T cell receptor(TCR alpha/beta)-T3 complex but transcribe TCR beta mRNA from either germ-line configuration (1/13) or partially (DJ) or fully (VDJ) rearranged (12/13) genes. These cells do not produce TCR alpha mRNA, but do contain T3 delta and T3 epsilon mRNA and accumulate T3 polypeptides, primarily in the perinuclear envelope. Equivalent normal T cells isolated from thymus have a predominantly germ-line configuration of TCR beta but contain intracellular T3 proteins. T3 gene expression is therefore a very early event in T cell differentiation. TCR alpha chain production appears to be the limiting maturation-linked event in the transport, assembly, and cell surface membrane insertion of the TCR alpha/beta-T3 complex.
对应于胸腺内T细胞分化最早可识别阶段的人类白血病细胞缺乏T细胞受体（TCRα/β）-T3复合物的细胞表面表达，但可从种系构型（1/13）、部分（DJ）或完全（VDJ）重排（12/13）的基因转录TCRβmRNA。这些细胞不产生TCRαmRNA，但确实含有T3δ和T3εmRNA，并积累T3多肽，主要在核周包膜中。从胸腺分离的等效正常T细胞具有主要为种系构型的TCRβ，但含有细胞内T3蛋白。因此，T3基因表达是T细胞分化中非常早期的事件。TCRα链的产生似乎是TCRα/β-T3复合物在运输、组装和细胞表面膜插入过程中与成熟相关的限制事件。
