Lucocq J M, Pryde J G, Berger E G, Warren G
J Cell Biol. 1987 Apr;104(4):865-74. doi: 10.1083/jcb.104.4.865.
Galactosyltransferase, a marker for trans-Golgi cisternae in interphase cells, was localized in mitotic HeLa cells embedded in Lowicryl K4M by immunoelectron microscopy. Specific labeling was found only over multivesicular structures that we term Golgi clusters. Unlike Golgi stacks in interphase cells, these clusters lacked elongated cisternae and ordered stacking of their components but did comprise two distinct regions, one containing electron-lucent vesicles and the other, smaller, vesiculo-tubular structures. Labeling for galactosyltransferase was found predominantly over the latter region. Both structures were embedded in a dense matrix that excluded ribosomes and the cluster was often bounded by cisternae of the rough endoplasmic reticulum, sometimes on all sides. Clusters were present at all stages of mitosis examined, which included prometaphase, metaphase, and telophase. They were also identified in conventionally processed mitotic cells and shown to contain another trans-Golgi marker, thiamine pyrophosphatase. Serial sectioning showed that clusters were discrete and globular and multiple copies appeared to be dispersed in the cytoplasm. Their possible role in the division of the Golgi apparatus is discussed.
半乳糖基转移酶是间期细胞中转高尔基体潴泡的一种标志物，通过免疫电子显微镜技术，它被定位在包埋于低温包埋剂K4M中的有丝分裂HeLa细胞内。仅在我们称为高尔基体簇的多囊泡结构上发现了特异性标记。与间期细胞中的高尔基体堆叠不同，这些簇缺乏细长的潴泡及其成分的有序堆叠，但确实包含两个不同的区域，一个区域含有电子透明的囊泡，另一个较小的区域是囊泡管状结构。半乳糖基转移酶的标记主要出现在后一个区域。这两种结构都包埋在一个排除核糖体的致密基质中，并且该簇常常被粗面内质网的潴泡包围，有时是全方位包围。在所检查的有丝分裂的各个阶段，包括前中期、中期和末期，都存在这些簇。它们也在常规处理的有丝分裂细胞中被鉴定出来，并显示含有另一种反式高尔基体标志物硫胺焦磷酸酶。连续切片显示这些簇是离散的、球状的，并且多个副本似乎分散在细胞质中。文中讨论了它们在高尔基体分裂中可能发挥的作用。
