College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Efficient and Clean Utilization of Manganese Resources, Central South University, Changsha, 410083, China.
Mikrochim Acta. 2019 May 1;186(5):316. doi: 10.1007/s00604-019-3412-y.
The authors report a sensitive electrochemical aptamer-based assay for the cancer biomarker human epidermal growth factor receptor-2 (HER2). It is based on the use of MnO nanosheets that were functionalized with phosphate and a HER2 binding aptamer and serve as the electrochemical probe. The assay follows a sandwich protocol. A peptide that can specifically recognize HER2 was immobilized on a gold electrode for the capture of HER2. Then, the functionalized MnO nanosheets were linked to the electrode via the binding between HER2 and HER2 aptamer on the MnO nanosheets. The reaction of phosphate and aptamer on the MnO nanosheets with molybdate leads to the formation of redox-active molybdophosphate. This results in dual signal amplification. The generated electrochemical current was measured at 0.22 V (vs. Ag/AgCl). The assay allows HER2 to be determined in the 0.1 to 500 pg·mL concentration range, and the detection limit is as low as 0.05 pg·mL. The assay was successfully applied for the detection of HER2 in spiked human serum samples. Graphical abstract Electrochemical detection of breast cancer biomarker human epidermal growth factor receptor-2 (HER2) is reported utilizing phosphate ions and aptamer functionalized MnO nanosheet as probe.
作者报道了一种基于适配体的灵敏电化学分析方法，用于检测癌症生物标志物人表皮生长因子受体-2（HER2）。该方法基于使用磷酸化和 HER2 结合适配体功能化的 MnO 纳米片作为电化学探针。该测定采用三明治式方案。一种能够特异性识别 HER2 的肽被固定在金电极上，用于捕获 HER2。然后，功能化的 MnO 纳米片通过 MnO 纳米片上的 HER2 和 HER2 适配体之间的结合与电极相连。MnO 纳米片上的磷酸和适配体与钼酸盐的反应导致形成具有氧化还原活性的钼磷酸盐。这导致双重信号放大。在 0.22 V（相对于 Ag/AgCl）下测量产生的电化学电流。该测定法允许在 0.1 至 500 pg·mL 的浓度范围内测定 HER2，检测限低至 0.05 pg·mL。该测定法成功地应用于检测人血清样本中 HER2 的含量。
