喀麦隆南部克里比农村登革热病毒 1 型爆发的调查：一项横断面研究。
Investigation of an Outbreak of Dengue Virus Serotype 1 in a Rural Area of Kribi, South Cameroon: A Cross-Sectional Study.
机构信息
National Reference Laboratory for Chikungunya and Dengue, Department of Virology, Centre Pasteur of Cameroon, Yaoundé, Cameroon.
Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.
出版信息
Intervirology. 2018;61(6):265-271. doi: 10.1159/000499465. Epub 2019 May 2.
BACKGROUND
On May 2017, a case of dengue serotype 1 was detected and confirmed through routine surveillance in a traveler returning from Kribi, a seaside town of Southern Cameroon. This study aimed at confirming the circulation of dengue virus (DENV) in Southern Cameroon.
METHODS
A cross sectional study was carried out in Londji near Kribi from June 21-25, 2017, by a joint team of Centre Pasteur of Cameroon and the Department of Diseases, Epidemics and Pandemics Control. Blood samples of consented participants were collected and tested for anti-D ENV IgM using an IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA), and for the detection of Zika, dengue, or chikungunya viruses using Trioplex real-time reverse transcription-polymerase chain reaction (RT-PCR). DENV RNA-positive samples were serotyped using an end-point nested RT-PCR.
RESULTS
Ninety-one participants were enrolled, 50.55% (46/91) of them males. The mean age of the population was 30.71 years (±18.89). In total, 14.28% (13/91) of the participants had DENV infection (3 anti-DENV IgM positive and 10 DENV serotype 1 RT-PCR positive).
CONCLUSION
The detection of DENV serotype 1 in an autochthonous population during this survey is a confirmation that the seaside city of Kribi is a risk area for contracting dengue infection in Cameroon.
背景
2017 年 5 月，通过对一名从喀麦隆南部滨海城镇克里比返回的旅行者的常规监测，发现并确认了一例 1 型登革热病例。本研究旨在确认登革热病毒（DENV）在喀麦隆南部的传播情况。
方法
2017 年 6 月 21 日至 25 日，喀麦隆巴斯德中心和疾病、流行病和大流行病控制司的联合小组在克里比附近的隆吉进行了一项横断面研究。采集同意参与的参与者的血液样本，使用 IgM 抗体捕获酶联免疫吸附试验（MAC-ELISA）检测抗 DENV IgM，并使用 Trioplex 实时逆转录聚合酶链反应（RT-PCR）检测寨卡、登革热或基孔肯雅热病毒。使用终点嵌套 RT-PCR 对 DENV RNA 阳性样本进行血清分型。
结果
共纳入 91 名参与者，其中 50.55%（46/91）为男性。人群的平均年龄为 30.71 岁（±18.89）。共有 14.28%（13/91）的参与者存在 DENV 感染（3 例抗 DENV IgM 阳性，10 例 DENV 血清型 1 RT-PCR 阳性）。
结论
在本次调查中，在当地人群中检测到 DENV 血清型 1，证实滨海城市克里比是喀麦隆感染登革热的风险地区。
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