Chandrashekar Rajan, Rai Mohandas, Kalal Bhuvanesh Sukhlal
Department of Pharmacology, A. J. Institute of Medical Sciences and Research Centre Mangaluru 575004, Karnataka, India.
A. J. Research Centre, A. J. Institute of Medical Sciences & Research Centre Mangaluru 575004, Karnataka, India.
Int J Biochem Mol Biol. 2022 Aug 20;13(4):40-48. eCollection 2022.
To evaluate the safe dose range of and ethanolic leaf extracts of acute and chronic oral toxicity study in Swiss Albino mice.
The Organization for Economic Co-operation and Development guideline was used for the toxicity studies. C. viscosum and L. indica plant extract were administered orally in a single dose of 2000 mg/kg, and general behavior, adverse effects, and mortality were studied for 72 h. For the chronic toxicity study, both plant extracts were administered orally to a separate set of animals at 300 mg/kg doses for 90 days. Animals body weight was taken out, blood and gastric juice were collected for biochemical parameters, and vital organs were collected for histopathological studies after sacrificing test and control group animals.
Both in acute and chronic toxicity assay, there was no significant alteration in body weight, physical signs, symptoms, hematological, biochemical parameters, and body organ weights compared to the normal group. The liver, kidney, and stomach histology did not show any drug-induced lesion.
The result indicates that the oral administration of and ethanolic plant extract did not cause any toxicological effects. Hence it could be regarded as a safe natural product for therapeutic use.
评估在瑞士白化小鼠中进行急性和慢性口服毒性研究时，[植物名称1]和[植物名称2]乙醇叶提取物的安全剂量范围。
毒性研究采用经济合作与发展组织的指导方针。[植物名称1]和[植物名称2]植物提取物以2000毫克/千克的单剂量口服给药，并对一般行为、不良反应和死亡率进行72小时的研究。对于慢性毒性研究，将两种植物提取物以300毫克/千克的剂量分别口服给予另一组动物，持续90天。在处死试验组和对照组动物后，记录动物体重，采集血液和胃液用于生化参数检测，并采集重要器官用于组织病理学研究。
与正常组相比，在急性和慢性毒性试验中，体重、体征、症状、血液学、生化参数及身体器官重量均无显著变化。肝脏、肾脏和胃的组织学检查未显示任何药物诱导的病变。
结果表明，口服[植物名称1]和[植物名称2]乙醇植物提取物未引起任何毒理学效应。因此，它可被视为一种安全的治疗用天然产物。
