Becaro Aline A, Siqueira Maria Célia, Puti Fernanda C, de Moura Marcia Regina, Correa Daniel S, Marconcini José Manoel, Mattoso Luiz H C, Ferreira Marcos David
Programa de Pós-Graduação em Biotecnologia (PPGBiotec), Centro de Ciências Exatas e Tecnologia, Universidade Federal de São Carlos (UFSCar), São Carlos, SP, 13565-905, Brazil.
Laboratório Nacional de Nanotecnologia para o Agronegócio (LNNA), EMBRAPA Instrumentação, São Carlos, SP, 13560-970, Brazil.
Environ Monit Assess. 2017 Jul;189(7):352. doi: 10.1007/s10661-017-6062-8. Epub 2017 Jun 24.
Several mutagenic agents may be present in substances released in the environment, which may cause serious environmental impacts. Among these substances, there is a special concern regarding the widespread use of silver nanoparticles (AgNP) in several products due to their widely known bactericidal properties, including in the medical field and the food industry (e.g., active packaging). The assessment of the effects of AgNP released in the environment, having different concentrations, sizes, and being associated or not to other types of materials, including polymers, is therefore essential. In this research, the objective was to evaluate the genotoxic and cytotoxic effects of AgNP (size range between 2 and 8 nm) on root meristematic cells of Allium cepa (A. cepa). Tests were carried out in the presence of colloidal solution of AgNP and AgNP mixed with carboxymethylcellulose (CMC), using distinct concentrations of AgNP. As a result, when compared to control samples, AgNP induced a mitotic index decrease and an increase of chromosomal aberration number for two studied concentrations. When AgNP was in the presence of CMC, no cytotoxic potential was verified, but only the genotoxic potential for AgNP dispersion having concentration of 12.4 ppm.
环境中释放的物质可能存在几种诱变剂，这可能会造成严重的环境影响。在这些物质中，由于银纳米颗粒（AgNP）具有广为人知的杀菌特性，包括在医学领域和食品工业（如活性包装）中的广泛应用，人们对其在多种产品中的广泛使用尤为关注。因此，评估不同浓度、尺寸且与包括聚合物在内的其他类型材料相关或不相关的环境中释放的AgNP的影响至关重要。在本研究中，目的是评估AgNP（尺寸范围在2至8纳米之间）对洋葱（A. cepa）根分生细胞的遗传毒性和细胞毒性作用。使用不同浓度的AgNP，在AgNP胶体溶液以及AgNP与羧甲基纤维素（CMC）混合的情况下进行测试。结果表明，与对照样品相比，对于两个研究浓度，AgNP导致有丝分裂指数降低和染色体畸变数量增加。当AgNP与CMC共存时，未证实有细胞毒性潜力，但仅对于浓度为12.4 ppm的AgNP分散体有遗传毒性潜力。
