Gastrointestinal and Anorectal Surgery, Changyi People's Hospital of Shandong Province, Changyi, China.
General Internal Medicine and Hematology, Changyi People's Hospital of Shandong Province, Changyi, China.
Mol Genet Genomic Med. 2019 Jun;7(6):e711. doi: 10.1002/mgg3.711. Epub 2019 May 2.
Long noncoding RNAs (lncRNAs) are from the family of noncoding RNAs. Existing studies have shown that lncRNAs are involved in many biological processes and are strongly related to the occurrence and development of tumors. Recent studies have indicated that lncRNA GIHCG participates in the progression of many cancers by adjusting cell proliferation and migration. Gastric cancer (GC) is a prevalent malignant tumor that arises from gastric epithelium. This study mainly explored the influence of GIHCG on GC and its underlying mechanism.
GIHCG expression was detected in GC through quantitative real-time polymerase chain reaction while the relationship of GIHCG with miR-1281 and miR-1281 with TLE1 was verified using dual luciferase reporter gene assay. The influence of GIHCG, miR-1281 and TLE1 on cell function was verified using cell counting Kit-8 (CCK-8) and Transwell experiment.
In GC, GIHCG was significantly overexpressed and significantly increased cell proliferation and migration, with the possible mechanism of upregulatingTLE1 expression through adsorption of miR-1281.
Taken together, we revealed the role of GIHCG/miR-1281/TLE1 in GC and provided a new perspective.
长链非编码 RNA（lncRNAs）属于非编码 RNA 家族。现有研究表明，lncRNAs 参与了许多生物学过程，与肿瘤的发生和发展密切相关。最近的研究表明，lncRNA GIHCG 通过调节细胞增殖和迁移参与许多癌症的进展。胃癌（GC）是一种源自胃上皮的常见恶性肿瘤。本研究主要探讨了 GIHCG 对 GC 的影响及其潜在机制。
通过实时定量聚合酶链反应检测 GC 中的 GIHCG 表达，通过双荧光素酶报告基因检测验证 GIHCG 与 miR-1281 以及 miR-1281 与 TLE1 的关系。通过细胞计数试剂盒-8（CCK-8）和 Transwell 实验验证 GIHCG、miR-1281 和 TLE1 对细胞功能的影响。
在 GC 中，GIHCG 明显过表达，明显增加细胞增殖和迁移，其可能的机制是通过吸附 miR-1281 而上调 TLE1 表达。
综上所述，我们揭示了 GIHCG/miR-1281/TLE1 在 GC 中的作用，为其提供了新的视角。
