Department of Pharmacokinetics and Biopharmaceutics, Institute of Biomedical Sciences, Tokushima University, 1-78-1, Sho-machi, Tokushima 770-8505, Japan.
School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Subang Jaya Selangor 47500, Malaysia.
Molecules. 2019 Apr 30;24(9):1689. doi: 10.3390/molecules24091689.
Intracellular polysulfide could regulate the redox balance via its anti-oxidant activity. However, the existence of polysulfide in biological fluids still remains unknown. Recently, we developed a quantitative analytical method for polysulfide and discovered that polysulfide exists in plasma and responds to oxidative stress. In this study, we confirmed the presence of polysulfide in other biological fluids, such as semen and nasal discharge. The levels of polysulfide in these biological fluids from healthy volunteers ( = 9) with identical characteristics were compared. Additionally, the circadian rhythm of plasma polysulfide was also investigated. The polysulfide levels detected from nasal discharge and seminal fluid were approximately 400 and 600 μM, respectively. No correlation could be found between plasma polysulfide and the polysulfide levels of tear, saliva, and nasal discharge. On the other hand, seminal polysulfide was positively correlated with plasma polysulfide, and almost all polysulfide contained in semen was found in seminal fluid. Intriguingly, saliva and seminal polysulfide strongly correlated with salivary amylase and sperm activities, respectively. These results provide a foundation for scientific breakthroughs in various research areas like infertility and the digestive system process.
细胞内多硫化物可以通过其抗氧化活性来调节氧化还原平衡。然而，生物液中的多硫化物的存在仍然未知。最近，我们开发了一种多硫化物的定量分析方法，发现多硫化物存在于血浆中，并对氧化应激有反应。在这项研究中，我们证实了多硫化物存在于其他生物液中，如精液和鼻分泌物。比较了来自具有相同特征的 9 名健康志愿者的这些生物液中的多硫化物水平。此外，还研究了血浆多硫化物的昼夜节律。从鼻分泌物和精液中检测到的多硫化物水平分别约为 400 和 600 μM。血浆多硫化物与泪液、唾液和鼻分泌物中的多硫化物水平之间没有相关性。另一方面，精液中的多硫化物与血浆多硫化物呈正相关，并且几乎所有存在于精液中的多硫化物都存在于精液中。有趣的是，唾液和精液中的多硫化物分别与唾液淀粉酶和精子活性密切相关。这些结果为生育力和消化系统过程等各个研究领域的科学突破提供了基础。
