Department of Gastroenterological Surgery, Yokohama City University, Yokohama City University, Yokohama, Kanagawa
Axcelead Drug Discovery Partners Inc., Fujisawa, Kanagawa, Japan
J Int Med Res. 2021 Nov;49(11):3000605211059936. doi: 10.1177/03000605211059936.
Sulphur-containing compounds have been linked to colorectal cancer by factors such as the presence of methyl mercaptan in intestinal gas and long-term dietary intake associated with sulphur-metabolizing microbiota. Therefore, this current case report hypothesized that active sulphur metabolism in colorectal cancer results in the formation of sulphur compounds in the intestine and, thus, examined sulphur metabolites possibly associated with sulphur respiration in colon cancer tissues. The patient was a 73-year-old female that underwent laparoscopic right hemicolectomy for ascending colon cancer. During the surgery, colon cancer tissues and normal intestinal mucosa samples were collected. After optimizing the sample concentrations for homogenization (pre-treatment), the samples were stabilized using a hydroxyphenyl-containing derivative and the relevant metabolites were quantified using liquid chromatography with tandem mass spectrometry. The results showed that cysteine persulfide and cysteine trisulfide levels were higher in colon cancer tissues than in normal mucosal tissues. Thus, sulphur metabolism, possibly sulphur respiration, is enhanced in colon cancer tissues.
含硫化合物与结直肠癌之间存在关联，其关联因素包括肠道气体中存在甲硫醇以及与硫代谢微生物群相关的长期饮食摄入。因此，本病例报告假设结直肠癌细胞中的活性硫代谢导致肠道中形成硫化合物，并因此检测了可能与结肠癌组织中硫呼吸相关的硫代谢物。患者为一名 73 岁女性，因升结肠癌接受腹腔镜右半结肠切除术。手术过程中，收集结肠癌组织和正常肠黏膜样本。在优化用于均质化的样品浓度（预处理）后，使用含羟苯基的衍生物稳定样品，并使用液相色谱-串联质谱法定量相关代谢物。结果表明，结肠癌组织中的半胱氨酸过硫化物和半胱氨酸三硫化物水平高于正常黏膜组织。因此，结肠癌组织中的硫代谢（可能是硫呼吸）增强。
