Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, Telangana, India.
Central Sericultural Research and Training Institute, Central Silk Board, NH-1A, Gallandar, Pampore, 192121, J&K, India.
Sci Rep. 2019 May 7;9(1):7012. doi: 10.1038/s41598-019-43332-1.
Aldehyde dehydrogenase (ALDH) carries out oxidation of toxic aldehydes using NAD/NADP as cofactors. In the present study, we performed a genome-wide identification and expression analysis of genes in the ALDH gene family in Brassica rapa. A total of 23 ALDH genes in the superfamily have been identified according to the classification of ALDH Gene Nomenclature Committee (AGNC). They were distributed unevenly across all 10 chromosomes. All the 23 Brassica rapa ALDH (BrALDH) genes exhibited varied expression patterns during treatments with abiotic stress inducers and hormonal treatments. The relative expression profiles of ALDH genes in B. rapa showed that they are predominantly expressed in leaves and stem suggesting their function in the vegetative tissues. BrALDH7B2 showed a strong response to abiotic stress and hormonal treatments as compared to other ALDH genes; therefore, it was overexpressed in heterologous hosts, E. coli and yeast to study its possible function under abiotic stress conditions. Over-expression of BrALDH7B2 in heterologous systems, E. coli and yeast cells conferred significant tolerance to abiotic stress treatments. Results from this work demonstrate that BrALDH genes are a promising and untapped genetic resource for crop improvement and could be deployed further in the development of drought and salinity tolerance in B. rapa and other economically important crops.
醛脱氢酶（ALDH）利用 NAD/NADP 作为辅助因子氧化有毒醛。本研究对白菜型油菜 ALDH 基因家族的基因进行了全基因组鉴定和表达分析。根据醛脱氢酶基因命名委员会（AGNC）的分类，共鉴定出超家族中的 23 个 ALDH 基因。它们在所有 10 条染色体上的分布不均匀。在非生物胁迫诱导和激素处理过程中，所有 23 个白菜型油菜 ALDH（BrALDH）基因表现出不同的表达模式。ALDH 基因在白菜型油菜中的相对表达谱表明，它们主要在叶片和茎中表达，表明它们在营养组织中的功能。与其他 ALDH 基因相比，BrALDH7B2 对非生物胁迫和激素处理表现出强烈的反应；因此，它在异源宿主大肠杆菌和酵母中过表达，以研究其在非生物胁迫条件下的可能功能。BrALDH7B2 在异源系统、大肠杆菌和酵母细胞中的过表达赋予了对非生物胁迫处理的显著耐受性。这项工作的结果表明，BrALDH 基因是作物改良的一种很有前途且尚未开发的遗传资源，可进一步用于提高白菜型油菜和其他经济重要作物的耐旱性和耐盐性。
