Department of Chemical Engineering, National Research Tomsk Polytechnic University, Lenin Avenue 30, 634050 Tomsk, Russia.
Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Charles University, Albertov 6, 12843 Prague 2, Czech Republic.
Sensors (Basel). 2019 May 7;19(9):2103. doi: 10.3390/s19092103.
A new simple electrochemical immunosensor approach for the determination of antibodies to tick-borne encephalitis virus (TBEV) in immunological products was developed and tested. The assay is performed by detecting the silver reduction signal in the bioconjugates with antibodies (). Here, signal is read by cathodic linear sweep voltammetry (CLSV) through the detection of silver chloride reduction on a gold-carbon composite electrode (GCCE). Covalent immobilization of the antigen on the electrode surface was performed after thiolation and glutarization of the GCCE. Specific attention has been paid to the selection of conditions for stabilizing both the silver nanoparticles and their . A simple flocculation test with NaCl was used to select the concentration of antibodies, and the additional stabilizer bovine serum albumin (BSA) was used for preparation. The antibodies to TBEV were quantified in the range from 50 IU·mL to 1600 IU·mL, with a detection limit of 50 IU·mL. The coefficient of determination () is 0.989. The electrochemical immunosensor was successfully applied to check the quality of immunological products containing IgG antibodies to TBEV. The present work paves the path for a novel method for monitoring TBEV in biological fluids.
一种新的简单电化学免疫传感器方法被开发并测试，用于检测免疫制品中抗蜱传脑炎病毒（TBEV）的抗体。该测定通过检测与抗体结合的生物缀合物中的银还原信号来进行（）。在这里，通过在金-碳复合电极（GCCE）上检测氯化银还原，通过阴极线性扫描伏安法（CLSV）读取信号。抗原在 GCCE 巯基化和戊二醛化后被共价固定在电极表面上。特别注意选择稳定纳米银及其的条件（）。使用简单的氯化钠絮凝试验选择抗体浓度，并使用额外的稳定剂牛血清白蛋白（BSA）进行。TBEV 的抗体在 50 IU·mL 到 1600 IU·mL 的范围内被定量，检测限为 50 IU·mL。决定系数（）为 0.989。电化学免疫传感器成功地应用于检查含有 TBEV IgG 抗体的免疫制品的质量。本工作为监测生物体液中的 TBEV 开辟了新的方法途径。
