Xia Shujing, Zhang Lili, Cheng Fenggan, Feng Zhenqing, Lu Lungen
Department of Pathology, Nanjing Medical University, Nanjing 211166, China.
Key Laboratory of Antibody Technology of National Health Commission, Nanjing 211166, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Apr 30;39(4):387-393. doi: 10.12122/j.issn.1673-4254.2019.04.02.
To study the expression of PSMA7 and its effect on proliferation, invasion and migration of gastric cancer and subcutaneous tumorigenesis in nude mice. >and subcutaneous tumorigenesis in nude mice.
Specimens of tumor tissues and paired adjacent tissues were collected from 60 patients with gastric cancer for detecting the expression levels of PSMA7 using immunohistochemical method. Gastric cancer cell line SGC7901 was transfected with a lentiviral vector to inhibit PSMA7 expression, and the changes in cell proliferation and invasion were observed using cell counting kit-8 (CCK-8), clone formation assay and Transwell assay. A BALB/c mouse model bearing subcutaneous gastric cancer xenograft was established using SGC7901 cells with stable PSMA7 knockdown to assess the effect of low expression of PSMA7 on xenograft growth.
Gastric cancer tissues expressed significantly higher levels of PSMA7 than the paired adjacent tissues ( < 0.05). In SGC7901 cells, interference of PSMA7 expression significantly inhibited the cell proliferation and invasion ( < 0.05). In the tumor-bearing BALB/c mice, the xenografts derived from SGC7901 cells with PSMA7 expression interference showed significant growth suppression as compared with the control xenografts ( < 0.05).
PPSMA 7 is overexpressed in gastric cancer tissues, and PSMA7 knockdown inhibits the proliferation, invasion, migration and subcutaneous tumorigenesis of gastric cancer cells in nude mice.
研究PSMA7的表达及其对胃癌增殖、侵袭、迁移和裸鼠皮下成瘤的影响。
收集60例胃癌患者的肿瘤组织标本及配对的癌旁组织，采用免疫组织化学方法检测PSMA7的表达水平。用慢病毒载体转染胃癌细胞系SGC7901以抑制PSMA7表达，采用细胞计数试剂盒-8（CCK-8）、克隆形成实验和Transwell实验观察细胞增殖和侵袭的变化。用稳定敲低PSMA7的SGC7901细胞建立BALB/c小鼠皮下胃癌异种移植模型，评估PSMA7低表达对异种移植瘤生长的影响。
胃癌组织中PSMA7的表达水平显著高于配对的癌旁组织（<0.05）。在SGC7901细胞中，干扰PSMA7表达可显著抑制细胞增殖和侵袭（<0.05）。在荷瘤BALB/c小鼠中，与对照异种移植瘤相比，PSMA7表达受干扰的SGC7901细胞来源的异种移植瘤生长明显受到抑制（<0.05）。
PSMA7在胃癌组织中过表达，敲低PSMA7可抑制裸鼠胃癌细胞的增殖、侵袭、迁移及皮下成瘤。
