Asadikaram Gholamreza, Vakili Sina, Akbari Hamed, Kheirmand-Parizi Marjan, Sadeghi Erfan, Asiabanha Majid, Shahrokhi Nader
Professor, Neuroscience Research Center, Institute of Neuropharmacology AND Department of Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
PhD Candidate, Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Addict Health. 2018 Apr;10(2):123-130. doi: 10.22122/ahj.v10i2.531.
The present study was carried out aiming to investigate the effects of opium on some biochemical factors in diabetic and non-diabetic male and female rats.
This experimental study was carried out on 28 male and 28 female Wistar rats. The animals were divided into diabetic addicted (DA), diabetic non-addicted (DNA), non-diabetic addicted (NDA), and non-diabetic non-addicted (NDNA) groups of male and female. A double dose of opium was intraperitoneally administered to the addicted groups. Peripheral blood samples were collected to measure the creatinine, uric acid, cholesterol, triglycerides (TG), total protein, and albumin levels. Three-way analysis of variance (ANOVA) was used to compare the mean levels of biofactors among the study groups.
Cholesterol and total protein were significantly affected by opium and sex, but not diabetes condition, such that there was a decrease of cholesterol and total protein levels in opium-addicted rats compared to non-opium-addicted ones. However, uric acid, TG, albumin, and creatinine were not affected by opium and diabetes conditions.
Opium significantly decreased cholesterol and total protein levels. It could be deduced that the effects of opium on cholesterol and total protein are not sex-dependent, moreover, opium consumption may not have significant effects on biochemical factors in diabetic conditions.
本研究旨在探讨鸦片对糖尿病和非糖尿病雄性及雌性大鼠某些生化因子的影响。
本实验研究选取了28只雄性和28只雌性Wistar大鼠。动物被分为糖尿病成瘾组（DA）、糖尿病非成瘾组（DNA）、非糖尿病成瘾组（NDA）和非糖尿病非成瘾组（NDNA），每组又分为雄性和雌性。对成瘾组腹腔注射双倍剂量的鸦片。采集外周血样本以测量肌酐、尿酸、胆固醇、甘油三酯（TG）、总蛋白和白蛋白水平。采用三因素方差分析（ANOVA）比较各研究组生物因子的平均水平。
胆固醇和总蛋白受鸦片和性别的显著影响，但不受糖尿病状况的影响，与未成瘾大鼠相比，成瘾大鼠的胆固醇和总蛋白水平降低。然而，尿酸、TG、白蛋白和肌酐不受鸦片和糖尿病状况的影响。
鸦片显著降低胆固醇和总蛋白水平。可以推断，鸦片对胆固醇和总蛋白的影响不依赖于性别，此外，吸食鸦片可能对糖尿病状态下的生化因子没有显著影响。
