Department of Biochemistry, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, P.O.Box: 7515412578, Iran.
Korean J Physiol Pharmacol. 2011 Dec;15(6):327-32. doi: 10.4196/kjpp.2011.15.6.327. Epub 2011 Dec 27.
It has been shown that some opium derivatives promote cell death via apoptosis. This study was designed to examine the influence of opium addiction on brain and liver cells apoptosis in male and female diabetic and non-diabetic Wistar rats. This experimental study was performed on normal, opium-addicted, diabetic and diabetic opium-addicted male and female rats. Apoptosis was evaluated by TUNEL and DNA fragmentation assays. Results of this study showed that apoptosis in opium-addicted and diabetic opium-addicted brain and liver cells were significantly higher than the both normal and diabetic rats. In addition, we found that apoptosis in brain cells of opium-addicted and diabetic opium-addicted male rats were significantly higher than opium-addicted and diabetic opium-addicted female, whereas apoptosis in liver cells of opium-addicted and diabetic opium-addicted female rats were significantly higher than opium-addicted and diabetic opium-addicted male. Overall, these results indicate that opium probably plays an important role in brain and liver cells apoptosis, therefore, leading neurotoxicity and hepatotoxicity. These findings also in away possibly means that male brain cells are more susceptible than female and interestingly liver of females are more sensitive than males in induction of apoptosis by opium.
已经表明，一些鸦片衍生物通过细胞凋亡促进细胞死亡。本研究旨在探讨鸦片成瘾对雄性和雌性糖尿病和非糖尿病 Wistar 大鼠脑和肝细胞凋亡的影响。这项实验研究是在正常、成瘾、糖尿病和糖尿病成瘾的雄性和雌性大鼠中进行的。通过 TUNEL 和 DNA 片段化分析评估细胞凋亡。本研究结果表明，成瘾和糖尿病成瘾大鼠的脑和肝细胞凋亡明显高于正常和糖尿病大鼠。此外，我们发现，雄性成瘾和糖尿病成瘾大鼠的脑细胞凋亡明显高于成瘾和糖尿病成瘾的雌性大鼠，而成瘾和糖尿病成瘾的雌性大鼠的肝细胞凋亡明显高于成瘾和糖尿病成瘾的雄性大鼠。总的来说，这些结果表明鸦片可能在脑和肝细胞凋亡中发挥重要作用，从而导致神经毒性和肝毒性。这些发现也在某种程度上意味着，雄性大脑细胞比雌性更敏感，有趣的是，雌性的肝脏对鸦片诱导的凋亡比雄性更敏感。
