Ryseck R P, Walldorf U, Hoffmann T, Hovemann B
Nucleic Acids Res. 1987 Apr 24;15(8):3317-33. doi: 10.1093/nar/15.8.3317.
A comparison of gene structure, sequence, and transcription pattern of heat shock loci 93D of Drosophila melanogaster and 48B of Drosophila hydei has been performed. Both heat shock loci consist of an unique region that is flanked by an internally repetitive element. Different members of these elements are highly conserved, repeat unit length, however, and primary sequence diverged totally. Whereas the overall gene structure in both species is substantially related, sequence conservation is only observed at very few sites in the unique region. These represent primarily sequences that are identified as regulatory elements for faithful transcription and processing. The number and size of transcripts obtained from heat shock locus 48B in third instar larvae closely resembles the pattern of heat shock locus 93D. Thus their quite alike structure and transcription pattern suggest strongly a conserved hitherto unknown function.
对黑腹果蝇热休克基因座93D和海德氏果蝇热休克基因座48B的基因结构、序列及转录模式进行了比较。两个热休克基因座均由一个独特区域组成，该区域两侧为内部重复元件。这些元件的不同成员高度保守，但重复单元长度和一级序列完全不同。虽然两个物种的整体基因结构有很大关联，但仅在独特区域的极少数位点观察到序列保守性。这些主要是被鉴定为忠实转录和加工调控元件的序列。从三龄幼虫的热休克基因座48B获得的转录本数量和大小与热休克基因座93D的模式非常相似。因此，它们极为相似的结构和转录模式强烈表明存在一种迄今未知的保守功能。
