Peters F P, Lubsen N H, Walldorf U, Moormann R J, Hovemann B
Mol Gen Genet. 1984;197(3):392-8. doi: 10.1007/BF00329934.
We have previously isolated a 500 bp-long cDNA clone, NO9-15, which is derived from a nuclear transcript originating from the heat shock locus 2-48B of Drosophila hydei (Peters et al. 1982). Sequence analysis shows that this clone carries 4 complete copies and 1 partial copy of a 115 bp repeat unit. The repeats are closely homologous with a maximal sequence divergence of about 10%. The sequence does not contain an open reading frame. The genomic organization of heat shock locus 2-48B, as probed with the cloned cDNA sequence NO9-15, is highly polymorphic. Four different allelic arrangements have been found in different inbred strains. A number of genomic clones isolated from region 2-48B, both in phage lambda and in cosmid vectors, all differ in length, mainly due to varying numbers of the NO9-15 repeat unit. These differences are found primarily in the proximal region of the locus. The transcribed region of these clones includes the distal sequence flanking the NO9-15 repeat as well as the NO9-15 repeat itself. An oligo A stretch was found between the distal flanking sequence and the NO9-15 repeat region.
我们之前分离出了一个500bp长的cDNA克隆，即NO9-15，它源自黑腹果蝇热休克基因座2-48B的一个核转录本（彼得斯等人，1982年）。序列分析表明，该克隆携带了115bp重复单元的4个完整拷贝和1个部分拷贝。这些重复序列高度同源，最大序列差异约为10%。该序列不包含开放阅读框。用克隆的cDNA序列NO9-15探测热休克基因座2-48B的基因组结构，发现其具有高度多态性。在不同的近交系中发现了四种不同的等位基因排列。从2-48B区域分离出的许多基因组克隆，无论是在λ噬菌体还是粘粒载体中，长度都不同，主要是由于NO9-15重复单元的数量不同。这些差异主要出现在基因座的近端区域。这些克隆的转录区域包括NO9-15重复序列侧翼的远端序列以及NO9-15重复序列本身。在远端侧翼序列和NO9-15重复区域之间发现了一段寡聚A序列。
