Henan Provincial People's Hospital and People's Hospital of Henan University, Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China.
International Ocular Surface Research Center and Institute of Ophthalmology, Key Laboratory for Regenerative Medicine, Jinan University Medical School, Guangzhou, China.
Invest Ophthalmol Vis Sci. 2019 May 1;60(6):2038-2048. doi: 10.1167/iovs.18-26030.
To determine whether high fructose intake (HFI) influences the daily transcriptional clock rhythms of murine extraorbital lacrimal glands (ELGs).
Timed ELGs were collected from two groups of C57BL/6J mice subjected to a 12-hour light/12-hour dark (LD) cycle for 10 days; the first group received water-only feeding and the second received water with 15% fructose. Total RNA was extracted and subjected to RNA sequencing. A JTK_CYCLE algorithm and computational software were used to determine the periodicity, rhythmicity, and amplitude of the cycling transcripts. The rhythmic transcripts from different conditions were analyzed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database.
HFI feeding caused massive remodeling of the preexisting rhythmic genes in the normal control (NC)-fed ELGs. The induced transcripts in HFI-fed mice resulted in a profound reorganization of the coordinated transcriptional oscillations and KEGG pathways. Moreover, HFI feeding significantly altered the distribution of the KEGG pathways over an LD in the ELGs. Finally, we found that the ELGs have a robust core clock machinery and HFI feeding altered amplitude and the peak phase of clock gene transcriptional oscillation in ELGs.
Short-term HFI reprograms the daily transcriptomic oscillation of murine ELGs. This information may deepen our understanding of the outcomes of lacrimal glands altered by nutritional challenge.
确定高果糖摄入（HFI）是否会影响小鼠眼外泪腺（ELG）的日常转录时钟节律。
从两组 C57BL/6J 小鼠的定时 ELG 中收集，这些小鼠在 10 天内经历了 12 小时光照/12 小时黑暗（LD）循环；第一组仅接受水喂养，第二组接受水和 15%果糖。提取总 RNA 并进行 RNA 测序。使用 JTK_CYCLE 算法和计算软件来确定周期性、节律性和循环转录物的幅度。使用京都基因与基因组百科全书（KEGG）数据库分析来自不同条件的节律性转录物。
HFI 喂养导致正常对照（NC）喂养的 ELG 中原有的节律性基因发生大规模重塑。HFI 喂养诱导的转录物导致协调的转录振荡和 KEGG 途径发生深刻的重组。此外，HFI 喂养显着改变了 ELGs 中 KEGG 途径在 LD 上的分布。最后，我们发现 ELG 具有强大的核心时钟机制，而 HFI 喂养改变了 ELG 中时钟基因转录振荡的幅度和峰相。
短期 HFI 重新编程了小鼠 ELG 的日常转录组振荡。这些信息可能加深我们对营养挑战改变泪腺的结果的理解。
