高脂肪摄入重塑了小鼠睑板腺的昼夜转录组图谱和代谢。
High-fat intake reshapes the circadian transcriptome profile and metabolism in murine meibomian glands.
作者信息
Zou Sen, Liu Jiangman, Si Hongli, Huang Duliurui, Qi Di, Pei Xiaoting, Lu Dingli, Huang Shenzhen, Li Zhijie
机构信息
Henan Eye Institute, Henan Eye Hospital and Henan Key Laboratory of Ophthalmology and Visual Science, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, China.
Department of Ophthalmology, People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Zhengzhou, China.
出版信息
Front Nutr. 2023 Mar 16;10:1146916. doi: 10.3389/fnut.2023.1146916. eCollection 2023.
BACKGROUND
Nutritional and food components reshape the peripheral clock and metabolism. However, whether food challenges affect the circadian clock and metabolism of meibomian glands (MGs) has not been fully explored. This study was designed to analyze alterations in the rhythmic transcriptome and metabolism of MGs of murine fed a balanced diet or a high-fat diet (HFD).
METHODS
Male C57BL/6J mice were maintained on a 12/12 h light/dark cycle and fed on normal chow (NC) or HFD for 4 weeks. MGs were collected from sacrificed animals at 3-h intervals throughout a 24-h circadian cycle. The circadian transcriptome of MGs was analyzed bioinformatics approaches using high-throughput RNA sequencing (RNA-seq). In addition, circadian oscillations of lipid components in MGs were analyzed.
RESULTS
Meibomian glands displayed robust transcriptome rhythmicity. HFD feeding significantly altered the circadian transcriptome profile of MGs-including composition and phase-and spatiotemporally affected the enriched signaling pathways. In addition, HFD feeding significantly altered the normal rhythmic oscillations of lipid components in MGs.
CONCLUSION
Our data show that HFD significantly affects MGs' rhythmicity, which reveals a high sensitivity of MGs' clocks to lipid composition in food.
背景
营养和食物成分会重塑外周生物钟和新陈代谢。然而，食物刺激是否会影响睑板腺（MGs）的昼夜节律和新陈代谢尚未得到充分研究。本研究旨在分析喂食均衡饮食或高脂饮食（HFD）的小鼠睑板腺的节律性转录组和新陈代谢的变化。
方法
雄性C57BL/6J小鼠维持12/12小时的光/暗循环，并喂食正常饲料（NC）或HFD 4周。在整个24小时的昼夜周期中，每隔3小时从处死后的动物身上收集睑板腺。使用高通量RNA测序（RNA-seq）通过生物信息学方法分析睑板腺的昼夜转录组。此外，还分析了睑板腺中脂质成分的昼夜振荡。
结果
睑板腺表现出强大的转录组节律性。高脂饮食喂养显著改变了睑板腺的昼夜转录组谱——包括组成和相位——并在时空上影响了富集的信号通路。此外，高脂饮食喂养显著改变了睑板腺中脂质成分的正常节律性振荡。
结论
我们的数据表明，高脂饮食显著影响睑板腺的节律性，这揭示了睑板腺生物钟对食物中脂质成分的高度敏感性。
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