Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 135 Songling Road, Qingdao 266101, P. R. China.
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet Bygning 220, 2800 Kgs, Lyngby, Denmark.
Nat Prod Rep. 2020 Jan 1;37(1):80-99. doi: 10.1039/c9np00016j. Epub 2019 May 10.
Covering: up to 2019As abundant natural products, isoprenoids have many useful industrial applications in the manufacturing of drugs, fragrances, food additives, colorants, rubber and advanced biofuels. The microbial production of isoprenoids has received much attention in recent years. Metabolic engineering approaches and synthetic biology have been utilized to reconstruct and optimize the metabolic pathways for isoprenoid production in cell factories. In this review, the recent advances in isoprenoid production using microbes are summarized, with a focus on MEP and MVA pathway engineering, downstream isoprenoid pathway engineering and microbial host engineering, which mainly includes central carbon pathway engineering. Finally, future perspectives for the improvement of isoprenoid production are discussed.
截至 2019 年
作为丰富的天然产物，类异戊二烯在药物、香料、食品添加剂、着色剂、橡胶和先进生物燃料的制造中有许多有用的工业应用。近年来，微生物生产类异戊二烯受到了广泛关注。代谢工程方法和合成生物学已被用于重建和优化细胞工厂中类异戊二烯生产的代谢途径。本综述重点介绍了利用微生物生产类异戊二烯的最新进展，包括 MEP 和 MVA 途径工程、下游类异戊二烯途径工程和微生物宿主工程，主要包括中心碳途径工程。最后，讨论了提高类异戊二烯生产的未来展望。
