Materials Science and Engineering, University of Florida, Gainesville, Florida.
Preventative and Restorative Dental Sciences, University of California San Francisco, San Francisco, California.
Orthod Craniofac Res. 2019 May;22 Suppl 1(Suppl 1):76-81. doi: 10.1111/ocr.12271.
Improved methods are needed to remineralize dentin caries in order to promote conservation of dentin tissue and minimize the surgical interventions that are currently required for clinical treatment. Here, we test the hypothesis that bulk substrates can be effectively mineralized via a dual analog system proposed by others, using a tripolyphosphate (TPP) "templating analog" and a poly(acrylic acid) (PAA) or poly(aspartic acid) (pAsp) "sequestration analog," the latter of which generates the polymer-induced liquid-precursor (PILP) mineralization process studied in our laboratory.
MATERIAL & METHODS: Demineralized human dentin slices were remineralized with and without pre-treatment with TPP, using either PAA or pAsp as the PILP process-directing agent. A control experiment with no polymer present was used for comparison.
No mineralization was observed in any of the PAA groups. In both the pAsp and no polymer groups, TPP inhibited mineralization on the surfaces of the specimens but promoted mineralization within the interiors. Pre-treatment with TPP enhanced overall mineralization of the pAsp group. However, when analysed via TEM, regions with little mineral were still present.
Poly(acrylic acid) was unable to remineralize demineralized dentin slices under the conditions employed, even when pre-treated with TPP. However, pre-treatment with TPP enhanced overall mineralization of specimens that were PILP-remineralized using pAsp.
需要改进的方法来再矿化牙本质龋，以促进牙本质组织的保存，并最大限度地减少目前临床治疗所需的手术干预。在这里，我们通过其他人提出的双模拟系统来测试这样一个假设，即大块基质可以通过三偏磷酸盐（TPP）“模板模拟物”和聚（丙烯酸）（PAA）或聚（天冬氨酸）（pAsp）“螯合模拟物”来有效矿化，后一种物质会产生我们实验室研究的聚合物诱导的液体前体（PILP）矿化过程。
用人牙本质脱矿切片进行再矿化，用 TPP 进行预处理，用 PAA 或 pAsp 作为 PILP 过程导向剂。使用没有聚合物的对照实验进行比较。
在 PAA 组中均未观察到矿化。在 pAsp 和无聚合物组中，TPP 抑制了标本表面的矿化，但促进了内部的矿化。TPP 的预处理增强了 pAsp 组的整体矿化。然而，通过 TEM 分析，仍存在矿化程度较低的区域。
在实验条件下，即使经过 TPP 预处理，聚（丙烯酸）也无法再矿化脱矿牙本质切片。然而，TPP 的预处理增强了使用 pAsp 进行 PILP 矿化的标本的整体矿化。
