• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在深龋体外牙齿模型中使用羧甲基纤维素/无定形磷酸钙纳米复合物支架对脱矿牙本质进行仿生再矿化。

Biomimetic remineralization of demineralized dentine using scaffold of CMC/ACP nanocomplexes in an in vitro tooth model of deep caries.

作者信息

Chen Zhen, Cao Shansong, Wang Haorong, Li Yanqiu, Kishen Anil, Deng Xuliang, Yang Xiaoping, Wang Yinghui, Cong Changhong, Wang Huajun, Zhang Xu

机构信息

School of Stomatology, Hospital of Stomatology, Tianjin Medical University, Tianjin, PR China.

Discipline of Endodontics, Faculty of Dentistry, University of Toronto, Toronto, Canada.

出版信息

PLoS One. 2015 Jan 14;10(1):e0116553. doi: 10.1371/journal.pone.0116553. eCollection 2015.

DOI:10.1371/journal.pone.0116553
PMID:25587986
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4294661/
Abstract

Currently, it is still a tough task for dentists to remineralize dentine in deep caries. The aim of this study was to remineralize demineralized dentine in a tooth model of deep caries using nanocomplexes of carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP) based on mimicking the stabilizing effect of dentine matrix protein 1 (DMP1) on ACP in the biomineralization of dentine. The experimental results indicate that CMC can stabilize ACP to form nanocomplexes of CMC/ACP, which is able to be processed into scaffolds by lyophilization. In the single-layer collagen model, ACP nanoparticles are released from scaffolds of CMC/ACP nanocomplexes dissolved and then infiltrate into collagen fibrils via the gap zones (40 nm) to accomplish intrafibrillar mineralization of collagen. With this method, the completely demineralized dentine was partially remineralized in the tooth mode. This is a bottom-up remineralizing strategy based on non-classical crystallization theory. Since nanocomplexes of CMC/ACP show a promising effect of remineralization on demineralized dentine via biomimetic strategy, thereby preserving dentinal tissue to the maximum extent possible, it would be a potential indirect pulp capping (IPC) material for the management of deep caries during vital pulp therapy based on the concept of minimally invasive dentistry (MID).

摘要

目前，对于牙医来说，使深龋中的牙本质再矿化仍然是一项艰巨的任务。本研究的目的是基于模拟牙本质基质蛋白1（DMP1）在牙本质生物矿化过程中对无定形磷酸钙（ACP）的稳定作用，使用羧甲基壳聚糖/无定形磷酸钙（CMC/ACP）纳米复合物在深龋牙齿模型中使脱矿牙本质再矿化。实验结果表明，CMC可以稳定ACP形成CMC/ACP纳米复合物，该复合物能够通过冻干加工成支架。在单层胶原蛋白模型中，ACP纳米颗粒从溶解的CMC/ACP纳米复合物支架中释放出来，然后通过间隙区域（40nm）渗入胶原纤维，完成胶原纤维内矿化。通过这种方法，在牙齿模型中，完全脱矿的牙本质被部分再矿化。这是一种基于非经典结晶理论的自下而上的再矿化策略。由于CMC/ACP纳米复合物通过仿生策略对脱矿牙本质显示出有前景的再矿化效果，从而最大程度地保留牙本质组织，基于微创牙科（MID）的概念，它将是牙髓病治疗中用于处理深龋的一种潜在的间接盖髓（IPC）材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/bd2346c5e213/pone.0116553.g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/57a8b81334fe/pone.0116553.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/c9e059b2ad67/pone.0116553.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/db6d93ddeb8b/pone.0116553.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/4065cf1b2cd8/pone.0116553.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/6da3ec71a4a3/pone.0116553.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/0684d0d0d519/pone.0116553.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/98a22f3d57f1/pone.0116553.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/7d93ac63cabc/pone.0116553.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/c7dc6e253d67/pone.0116553.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/ad533c2d1316/pone.0116553.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/bd2346c5e213/pone.0116553.g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/57a8b81334fe/pone.0116553.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/c9e059b2ad67/pone.0116553.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/db6d93ddeb8b/pone.0116553.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/4065cf1b2cd8/pone.0116553.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/6da3ec71a4a3/pone.0116553.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/0684d0d0d519/pone.0116553.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/98a22f3d57f1/pone.0116553.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/7d93ac63cabc/pone.0116553.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/c7dc6e253d67/pone.0116553.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/ad533c2d1316/pone.0116553.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f189/4294661/bd2346c5e213/pone.0116553.g011.jpg

相似文献

1
Biomimetic remineralization of demineralized dentine using scaffold of CMC/ACP nanocomplexes in an in vitro tooth model of deep caries.在深龋体外牙齿模型中使用羧甲基纤维素/无定形磷酸钙纳米复合物支架对脱矿牙本质进行仿生再矿化。
PLoS One. 2015 Jan 14;10(1):e0116553. doi: 10.1371/journal.pone.0116553. eCollection 2015.
2
Rapid biomimetic remineralization of the demineralized enamel surface using nano-particles of amorphous calcium phosphate guided by chimaeric peptides.利用嵌合肽引导的无定形磷酸钙纳米颗粒快速仿生再矿化脱矿牙釉质表面。
Dent Mater. 2017 Nov;33(11):1217-1228. doi: 10.1016/j.dental.2017.07.015. Epub 2017 Jul 31.
3
Biomimetic deposition of calcium phosphate minerals on the surface of partially demineralized dentine modified with phosphorylated chitosan.仿生沉积磷酸钙矿物质于经磷酸化壳聚糖改性的部分脱矿牙本质表面。
J Biomed Mater Res B Appl Biomater. 2011 Jul;98(1):150-9. doi: 10.1002/jbm.b.31844. Epub 2011 May 2.
4
Synergistic intrafibrillar/extrafibrillar mineralization of collagen scaffolds based on a biomimetic strategy to promote the regeneration of bone defects.基于仿生策略的胶原蛋白支架协同纤维内/纤维外矿化以促进骨缺损再生
Int J Nanomedicine. 2016 May 12;11:2053-67. doi: 10.2147/IJN.S102844. eCollection 2016.
5
Biomimetic mineralisation of phosphorylated dentine by CPP-ACP.酪蛋白磷酸肽-无定形磷酸钙对磷酸化牙本质的仿生矿化作用
J Dent. 2013 Sep;41(9):818-25. doi: 10.1016/j.jdent.2013.06.008. Epub 2013 Jun 25.
6
Promotion Effect of Carboxymethyl Chitosan on Dental Caries via Intrafibrillar Mineralization of Collagen and Dentin Remineralization.羧甲基壳聚糖通过胶原纤维内矿化和牙本质再矿化对龋齿的促进作用
Materials (Basel). 2022 Jul 12;15(14):4835. doi: 10.3390/ma15144835.
7
Oriented and Ordered Biomimetic Remineralization of the Surface of Demineralized Dental Enamel Using HAP@ACP Nanoparticles Guided by Glycine.基于甘氨酸引导的 HAP@ACP 纳米颗粒对脱矿牙釉质表面进行定向有序仿生再矿化
Sci Rep. 2017 Jan 12;7:40701. doi: 10.1038/srep40701.
8
MV-mimicking micelles loaded with PEG-serine-ACP nanoparticles to achieve biomimetic intra/extra fibrillar mineralization of collagen in vitro.载聚乙二醇丝氨酸-ACP 纳米粒的 MV 模拟胶束实现胶原纤维内/外仿生矿化。
Biochim Biophys Acta Gen Subj. 2019 Jan;1863(1):167-181. doi: 10.1016/j.bbagen.2018.10.005. Epub 2018 Oct 10.
9
Use of experimental-resin-based materials doped with carboxymethyl chitosan and calcium phosphate microfillers to induce biomimetic remineralization of caries-affected dentin.使用载有羧甲基壳聚糖和磷酸钙微填料的实验性树脂基材料诱导龋损牙本质的仿生再矿化。
J Mech Behav Biomed Mater. 2019 Jan;89:81-88. doi: 10.1016/j.jmbbm.2018.09.008. Epub 2018 Sep 7.
10
Biomimetic remineralization of demineralized enamel with nano-complexes of phosphorylated chitosan and amorphous calcium phosphate.磷酸化壳聚糖与无定形磷酸钙纳米复合物对脱矿牙釉质的仿生再矿化
J Mater Sci Mater Med. 2014 Dec;25(12):2619-28. doi: 10.1007/s10856-014-5285-2. Epub 2014 Jul 30.

引用本文的文献

1
Re-Mineralization By Polyelectrolyte Nanocomposite For Effective Against Dentin Hypersensitivity.用于有效对抗牙本质过敏的聚电解质纳米复合材料再矿化
Int J Nanomedicine. 2025 Aug 15;20:9981-9998. doi: 10.2147/IJN.S530148. eCollection 2025.
2
Multifunctional quantum dot-decorated drug-loaded magnetite nanosystem for dual optical-magnetic resonance imaging and drug delivery.用于双光磁共振成像和药物递送的多功能量子点修饰的载药磁铁矿纳米系统
RSC Adv. 2025 Jun 23;15(27):21269-21283. doi: 10.1039/d5ra03379a.
3
Dose-dependent osteoimmunomodulatory effects of amorphous calcium phosphate nanoparticles promote 3D-printed scaffold-mediated bone regeneration.

本文引用的文献

1
Tertiary dentinogenesis with calcium hydroxide: a review of proposed mechanisms.三进制牙本质生成与氢氧化钙：作用机制的综述。
Int Endod J. 2013 Jan;46(1):3-19. doi: 10.1111/j.1365-2591.2012.02101.x. Epub 2012 Aug 13.
2
Functional remineralization of dentin lesions using polymer-induced liquid-precursor process.采用聚合物诱导液芯技术对牙本质病损进行功能再矿化。
PLoS One. 2012;7(6):e38852. doi: 10.1371/journal.pone.0038852. Epub 2012 Jun 13.
3
Novel microwave synthesis of amorphous calcium phosphate nanospheres.新型微波合成无定形磷酸钙纳米球。
无定形磷酸钙纳米颗粒的剂量依赖性骨免疫调节作用促进3D打印支架介导的骨再生。
Bioact Mater. 2025 May 13;51:197-210. doi: 10.1016/j.bioactmat.2025.05.010. eCollection 2025 Sep.
4
Next-Generation Biomaterials for Vital Pulp Therapy: Exploring Biological Properties and Dentin Regeneration Mechanisms.用于活髓治疗的下一代生物材料：探索生物学特性和牙本质再生机制
Bioengineering (Basel). 2025 Feb 28;12(3):248. doi: 10.3390/bioengineering12030248.
5
Nano Phosphorylated Pullulan-Integrated Carboxymethyl Chitosan Scaffold for Regeneration of the Pulp-Dentin Complex: An Study.用于牙髓-牙本质复合体再生的纳米磷酸化普鲁兰多糖整合羧甲基壳聚糖支架：一项研究。
J Pharm Bioallied Sci. 2024 Dec;16(Suppl 5):S4563-S4567. doi: 10.4103/jpbs.jpbs_1222_24. Epub 2025 Jan 30.
6
Effect of Bioactive Glass into Mineral Trioxide Aggregate on the Biocompatibility and Mineralization Potential of Dental Pulp Stem Cells.生物活性玻璃加入三氧化矿物凝聚体对牙髓干细胞生物相容性和矿化潜能的影响。
Biomater Res. 2025 Feb 7;29:0142. doi: 10.34133/bmr.0142. eCollection 2025.
7
Nanoparticles Induced Biomimetic Remineralization of Acid-Etched Dentin.纳米颗粒诱导酸蚀牙本质的仿生再矿化
J Dent (Shiraz). 2024 Dec 1;25(4):359-368. doi: 10.30476/dentjods.2024.98928.2117. eCollection 2024 Dec.
8
Unraveling Nanomaterials in Biomimetic Mineralization of Dental Hard Tissue: Focusing on Advantages, Mechanisms, and Prospects.解析仿生矿化牙体硬组织中的纳米材料：聚焦优势、机制与展望。
Adv Sci (Weinh). 2024 Oct;11(40):e2405763. doi: 10.1002/advs.202405763. Epub 2024 Aug 29.
9
Carboxymethyl chitosan stabilized AuNPs/ACP nanohybrids in enamel white spot lesions.羧甲基壳聚糖稳定的金纳米粒子/无定形磷酸钙纳米杂化物用于牙釉质白斑病变
Front Bioeng Biotechnol. 2024 Jul 16;12:1421887. doi: 10.3389/fbioe.2024.1421887. eCollection 2024.
10
Evaluation of Physical Properties in Carboxymethyl Chitosan Modified Glass Ionomer Cements and the Effect for Dentin Remineralization: SEM/EDX, Compressive Strength, and Ca/P Ratio.羧甲基壳聚糖改性玻璃离子水门汀物理性能评估及其对牙本质再矿化的影响：扫描电子显微镜/能谱仪、抗压强度和钙磷比
Eur J Dent. 2025 Feb;19(1):187-196. doi: 10.1055/s-0044-1786864. Epub 2024 Jul 16.
J Biomed Mater Res B Appl Biomater. 2012 May;100(4):1142-50. doi: 10.1002/jbm.b.32681. Epub 2012 Feb 14.
4
Remineralization of partially demineralized dentine substrate based on a biomimetic strategy.基于仿生策略的部分脱矿牙本质基质的再矿化。
J Mater Sci Mater Med. 2012 Mar;23(3):733-42. doi: 10.1007/s10856-012-4550-5. Epub 2012 Jan 21.
5
An ex vivo study to evaluate the remineralizing and antimicrobial efficacy of silver diamine fluoride and glass ionomer cement type VII for their proposed use as indirect pulp capping materials - Part I.一项体外研究，旨在评估氟化银氨和VII型玻璃离子水门汀作为间接盖髓材料的再矿化和抗菌效果——第一部分。
J Conserv Dent. 2011 Apr;14(2):113-6. doi: 10.4103/0972-0707.82603.
6
Amorphous calcium phosphate and its application in dentistry.无定形磷酸钙及其在牙科中的应用。
Chem Cent J. 2011 Jul 8;5:40. doi: 10.1186/1752-153X-5-40.
7
Biomimetic deposition of calcium phosphate minerals on the surface of partially demineralized dentine modified with phosphorylated chitosan.仿生沉积磷酸钙矿物质于经磷酸化壳聚糖改性的部分脱矿牙本质表面。
J Biomed Mater Res B Appl Biomater. 2011 Jul;98(1):150-9. doi: 10.1002/jbm.b.31844. Epub 2011 May 2.
8
Hierarchical and non-hierarchical mineralisation of collagen.胶原的分级和非分级矿化。
Biomaterials. 2011 Feb;32(5):1291-300. doi: 10.1016/j.biomaterials.2010.10.018. Epub 2010 Oct 30.
9
The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors.胶原在存在羟磷灰石成核抑制剂时对骨磷灰石形成的作用。
Nat Mater. 2010 Dec;9(12):1004-9. doi: 10.1038/nmat2875. Epub 2010 Oct 24.
10
Bioactivity of novel carboxymethyl chitosan scaffold incorporating MTA in a tooth model.新型羧甲基壳聚糖支架结合 MTA 在牙模型中的生物活性。
Int Endod J. 2010 Oct;43(10):930-9. doi: 10.1111/j.1365-2591.2010.01771.x. Epub 2010 Aug 3.