Department of Chemistry, Biology, and Marine Science, University of the Ryukyus, Nishihara-cho, Okinawa 903-2013, Japan.
Bioinspired Soft Matter Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-son, Okinawa 904-0495, Japan.
Fitoterapia. 2019 Jul;136:104162. doi: 10.1016/j.fitote.2019.05.001. Epub 2019 May 7.
Three new cembranolides (1-3) were isolated from an Okinawan soft coral, Lobophytum sp., together with the known cembranolide diterpenoids (4-9). Their structures were determined by extensive analysis of spectroscopic data (1D and 2D NMR, IR, and MS), molecular modeling, and comparison with data reported elsewhere. All compounds contain an α-methylene-γ-lactone ring adjacent to a cembrane, and some of them (1, 6-8) have an epoxide ring as well. The new metabolites were evaluated for cytotoxicity against HeLa, A459, B16-F10, and RAW 264.7 cells and anti-inflammatory effect in LPS-stimulated inflammatory RAW 264.7 macrophage cells.
从冲绳软珊瑚 Lobophytum sp. 中分离得到三个新的cembranolide（1-3），以及已知的cembranolide 二萜（4-9）。通过对光谱数据（1D 和 2D NMR、IR 和 MS）、分子建模以及与其他地方报道的数据进行广泛分析，确定了它们的结构。所有化合物都含有一个与cembrane 相邻的α-亚甲基-γ-内酯环，其中一些（1、6-8）还含有环氧环。评估了新代谢物对 HeLa、A459、B16-F10 和 RAW 264.7 细胞的细胞毒性以及对 LPS 刺激的炎症 RAW 264.7 巨噬细胞的抗炎作用。
