Burns Research Group, ANZAC Research Institute, Concord Hospital, University of Sydney, Concord West, NSW 2139, Australia.
Burns Research Group, ANZAC Research Institute, Concord Hospital, University of Sydney, Concord West, NSW 2139, Australia; Disciplines of Surgery, School of Medicine, University of Sydney, NSW 2006, Australia.
Burns. 2019 Nov;45(7):1639-1648. doi: 10.1016/j.burns.2019.04.014. Epub 2019 May 7.
The gold standard treatment for severe burn injuries is autologous skin grafting and the use of commercial dermal substitutes. However, resulting skin tissue following treatment usually displays abnormal morphology and functionality including scarring, skin contracture due to the poor elasticity and strength of existing dermal substitutes. In this study, we have developed a triple-polymer scaffold made of collagen-elastin-polycaprolactone (CEP) composite, aiming to enhance the mechanical properties of the scaffold while retaining its biological properties in promoting cell attachment, proliferation and tissue regeneration. The inclusion of elastin was revealed to decrease the stiffness of the scaffold, while also decreasing hysteresis and increasing elasticity. In mice, electrospun collagen-elastin-PCL scaffolds promoted keratinocyte and fibroblast proliferation, tissue integration and accelerated early-stage angiogenesis. Only a mild inflammatory response was observed in the first 2 weeks post-subcutaneous implantation. Our data indicates that the electrospun collagen-elastin-PCL scaffolds could potentially serve as a skin substitute to promote skin cell growth and tissue regeneration after severe burn injury.
严重烧伤的金标准治疗方法是自体皮肤移植和使用商业真皮替代物。然而，治疗后得到的皮肤组织通常表现出异常的形态和功能，包括瘢痕形成、由于现有真皮替代物弹性和强度差而导致的皮肤挛缩。在这项研究中，我们开发了一种由胶原蛋白-弹性蛋白-聚己内酯（CEP）复合材料制成的三聚物支架，旨在提高支架的机械性能，同时保留其促进细胞附着、增殖和组织再生的生物特性。弹性蛋白的加入降低了支架的刚度，同时也降低了滞后并增加了弹性。在小鼠中，静电纺丝胶原蛋白-弹性蛋白-PCL 支架促进角质形成细胞和成纤维细胞的增殖、组织整合和早期血管生成。在皮下植入后的前 2 周仅观察到轻微的炎症反应。我们的数据表明，静电纺丝胶原蛋白-弹性蛋白-PCL 支架可能可作为一种皮肤替代物，促进严重烧伤后皮肤细胞的生长和组织再生。
